common variable immunodeficiency (cvid) is a heterogeneous heritable disease characterized by arrest in b cell differentiation. an association between cvid and two hla haplotypes, haplotype i (hla-a1, hla-b8, hla-dr3) and haplotype ii (hla-a29, hla-b44, hladr7)has been previously documented. in the present study, we have attempted to find an association between susceptibility to cvid and hla cl...

analysis of  receptor–ligand  interactions  in  the  context  of  diseases  necessitates  to understand how hla–kir genotypes function in diseases. although cd56+  lymphocytes are derived from multiple lineages, they share a functional association with immunosurviellance and antimicrobial responses. the present study aimed to determine whether kir phenotype in cd56 lymphocytes and corresponding...

human leukocyte antigen-g which is an immune tolerance effecter molecule has an important role in the maintenance of fetus during pregnancy. abortion is one of the complications of pregnancy period.  in this research, we have studied levels of hla-g 5, hla-g7 isoforms in the abortion-threatened pregnant women in comparison with controls. in a case-control study, 101 abortion-threatened women an...

studies on hla-g, a nonpolymorphic antigen of non-classical hla class i, is of basic and clinical significance. in the present study, the expression of hla-g proteins in the human skin tissue sections of normal and autoimmune pemphigus vulgaris (pv) individuals were investigated using monoclonal antibodies. the antibodies recognized both membrane-bound and soluble isoforms of hla-g. rt-pcr was ...

serology and dna techniques are employed for human leukocyte antigen (hla) typing in different transplant centers. results may not always correlate well and may need retyping with different technique. all the patients (with aplastic anemia, thalassemia, and immunodeficiency) and their donors, requiring hla typing for bone marrow transplant were enrolled in the study. serological hla typing was ...

Background: HLA-G is a nonclassical HLA class I molecule which, when elevated in tumor cells, is one of the main factors involved in tumor evasion of immune responses including NK and T cells. Objective: To evaluate the effect of HLA-G downregulation on NK cell cytotoxicity in tumor cell lines. Methods: The expression level of HLA-G was measured by real-time PCR and flowcytometry after transfec...

膨大な数のHLA遺伝子型を判定するDNAタイピング（HLA DNAタイピング）は，HLA適合造血細胞移植の実施に欠かせない臨床検査技術である。2010年代に開発された次世代シークエンシング（NGS）技術に基づくHLA DNAタイピング（NGS-HLA）法は，遺伝子全長レベルで既知，新規およびnullアレルを効率よく検出する比較的新しいHLA DNAタイピング技術である。この技術は，現在の主流である高解像度HLA DNAタイピング法（現行法）よりもさらに正確なHLA多型情報が得られるため，造血細胞移植医療分野に新たな有益情報を提供する可能性がある。この優れたタイピング技術は，2020年3月から日本骨髄バンクを介した患者HLA確認検査やドナーHLAオプション検査に導入されており，コーディネート期間の短縮や移植成績の向上が期待されている。本稿では，造血細胞移植におけるNGS-HLA法の利点...

Mesenchymal stem cells (MSCs) are multipotent progenitor that reported to be immune-privileged and immune-evasive. MSCs capable of differentiating into specific cell types for subsequent use in cell-based therapy. They express low levels human leucocyte antigen (HLA)-ABC no HLA-DR. Wharton’s jelly-derived (WJ-MSCs) were also found leukocyte G (HLA-G), which renders them immunosuppressive. This ...

Background: Natural killer (NK) cells are the effector cells of innate immunity that respond to infection and tumor. Interactions between killer cell immunoglobulin like receptors (KIR) and human leukocyte antigen (HLA) class I molecules regulate NK cells responses to eliminate infected and transformed cells. Objective: To investigate the impact of KIR genes, HLA ligand genes, and KIR-HLA combi...