Automated sequence analysis of a 410-bp region of the axyR gene in 105 Mycobacterium tuberculosis complex isolates identified a polymorphic nucleotide that differentiated Mycobacterium bovis isolates from other complex members. All 29 M. bovis isolates sequenced had an adenine residue at nucleotide 285, whereas all 76 other complex isolates had a guanine residue. PCR-restriction fragment length...

In a previous study, katE complementation of an E. coli katG mutant suggested that an overexpression of katE was the cause for low survival after UV-A treatment. In this study, when JW strains lacked either katE or katG were complemented with katE, this overexpression was not observed. Rather, there were no differences between the two complemented JW strains after UV-A treatment. A possible the...

Cysteine residues, and in particular their thiolate groups, react not only with reactive oxygen species but also with electrophiles and with reactive nitrogen species. Thus, cysteine oxidation has often been linked to the toxic effects of some of these reactive molecules. However, thiol-based switches are common in protein sensors of antioxidant cascades, in both prokaryotic and eukaryotic orga...