Granule-mediated cytolysis is the major pathway for killer lymphocytes to kill pathogens and tumor cells. Little is known about how granzyme K functions in killer lymphocyte-mediated cytolysis. We previously showed that human GzmK triggers rapid cell death independently of caspase activation with single-stranded DNA nicks, similar to GzmA. In this study we found that GzmK can induce rapid react...

Justicia procumbens is a traditional Taiwanese herbal remedy used to treat fever, pain, and cancer. Justicidin A, isolated from Justicia procumbens, has been reported to suppress in vitro growth of several tumor cell lines as well as hepatoma cells. In this study, justicidin A activated caspase-8 to increase tBid, disrupted mitochondrial membrane potential (Delta psi(m)), and caused the release...

Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) is a homo-trimeric cytotoxic ligand. Several studies have demonstrated that incorporation of artificial trimerization motifs into the TRAIL protein leads to the enhancement of biological activity. Here, we show that linkage of the isoleucine zipper hexamerization motif to the N-terminus of TRAIL, referred as ILz(6):TRAIL, lea...

Multiple apoptotic pathways release cytochrome c from the mitochondrial intermembrane space, resulting in the activation of downstream caspases. In vivo activation of Fas (CD95) resulted in increased permeability of the mitochondrial outer membrane and depletion of cytochrome c stores. Serial measurements of oxygen consumption, NADH redox state and membrane potential revealed a loss of respirat...

صفحه‌­ی مینیاتوری LEU پرتودهی شده در رآکتور تحقیقاتی تهران که برای تولید رادیوایزوتوپ مولیبدن-99 استفاده می‌شود، منبع پرتوزایی متشکل از رادیوایزوتوپ‌های مختلف است. محاسبه و تخمین آهنگ دز تابش‌های گامای گسیلی این منبع، به منظور مقایسه با مقادیر مجاز توصیه حمل‌ونقل مواد پرتوزا حفاظت پرتویی کارکنان حین انجام تست‌های آزمایشگاهی داغ، پیش هر اقدام عملی، امری ضروری مقاله، اعتبار یک روش پیشنهادی جهت هد...