The NS3 protein of hepatitis C virus (HCV) has a serine protease activity in its N-terminal region, which plays a crucial role in virus replication. This region has also been reported to interact not only with its viral cofactor NS4A, but also with a number of host-cell proteins, which suggests a multifunctional feature of NS3. By means of yeast two-hybrid screening using an N-terminal region o...

The NS2B-NS3(pro) polyprotein segment from the dengue virus serotype 2 strain 16681 was purified from overexpressing E. coli by metal chelate affinity chromatography and gel filtration. Enzymatic activity of the refolded NS2B-NS3(pro) protease complex was determined in vitro with dansyl-labeled peptide substrates, based upon native dengue virus type 2 cleavage sites. The 12mer substrate peptide...

Antiviral immunity requires recognition of viral pathogens and activation of cytotoxic and Th cells by innate immune cells. In this study, we demonstrate that hepatitis C virus (HCV) core and nonstructural protein 3 (NS3), but not envelope 2 proteins (E2), activate monocytes and myeloid dendritic cells (DCs) and partially reproduce abnormalities found in chronic HCV infection. HCV core or NS3 (...

The hepatitis C virus (HCV) nonstructural 3 protein (NS3) contains at least two domains associated with multiple enzymatic activities; a serine protease activity resides in the N-terminal one-third of the protein, whereas RNA helicase activity and RNA-stimulated nucleoside triphosphatase activity are associated with the C-terminal portion. To study the possible mutual influence of these enzymat...

Background: The National Surveys of Sexual Attitudes and Lifestyles COVID study (Natsal-COVID) was designed to understand the impact COVID-19 on Britain’s sexual reproductive health (SRH). Natsal-COVID Wave 1 survey qualitative follow-up interviews were conducted in 2020. The 2 capture one-year prevalence estimates for key SRH outcomes measure changes over first year...

Background:Africa will miss the maternal and neonatal health (MNH) Sustainable Development Goals (SDGs) targets if current trajectory is followed. The African Academy of Sciences has formed an expert newborn group to discuss actions improve MNH SDG targets. team, among other recommendations, chose implement research prioritization exercise for Africa covering four gr...

Background: The recovery of phenolic compounds is seen as an arduous task because are available free aglycones, sugar or ester conjugates, polymers with several monomeric components. Furthermore, do not disperse evenly and may be connected to cell walls, carbohydrates, proteins. This study looks at the optimization factors that affect efficiency for extraction from s...

Background:Lead is a neurotoxic metal that crosses the placenta freely. It has adverse effects on range of birth outcomes. The few studies reporting associations prenatal exposure to lead and child growth have had conflicting results. This study aimed examine effect children’s from 4 61 months age.Methods:Pregnant women were enroll...

Background: The outbreak of the COVID-19 pandemic has affected education sector around world. In order to control spread virus, eLearning practice been introduced in Saudi higher education. Such online communication become an important tool narrow teaching gap. This study assessed characteristics and distance learning inclination Imam Abdulrahman BinFaisal University...

Background: Accurate viral load (VL) determination is paramount to determine the efficacy of anti-HIV-1 therapy. The conventional method used, fit-point (FP), assumes an equal efficiency in polymerase chain reaction (PCR) among samples that might not hold for low-input templates. An alternative approach, maxRatio, was introduced compensate inhibition PCR.<ns3...