In green-to-red photoconvertible fluorescent proteins, a three-ring chromophore is generated by the light-activated incorporation of a histidine residue into the conjugated π-system. We have determined the pH-rate profile and high- and low-pH X-ray structures of a least evolved ancestor (LEA) protein constructed in the laboratory based on statistical sequence analysis. LEA incorporates the mini...

BACKGROUND LEA (late embryogenesis abundant) proteins encode conserved N-terminal mitochondrial signal domains and C-terminal (A/TAEKAK) motif repeats, long-presumed to confer cell resistance to stress and death cues. This prompted the hypothesis that LEA proteins are central to mitochondria mechanisms that connect bioenergetics with cell responses to stress and death signaling. In support of t...

OBJECTIVE To determine the effect of contact with a podiatrist on the occurrence of Lower Extremity Amputation (LEA) in people with diabetes. DESIGN AND DATA SOURCES We conducted a systematic review of available literature on the effect of contact with a podiatrist on the risk of LEA in people with diabetes. Eligible studies, published in English, were identified through searches of PubMed, C...

Drought stress is a major factor limiting wheat growth and productivity. Late embryogenesis abundant (LEA) proteins are tolerant to water-related stress. To reveal the regulatory mechanisms of LEA proteins under drought stress, we cloned a novel group 3 LEA gene, namely, TaDlea3, from wheat (Triticum aestivum L.) Shaanhe 6. Subcellular localization assay showed that TaDlea3 protein accumulated ...

Black point of barley grain is a disorder characterised by a brown-black discolouration at the embryo end of the grain. Black point is undesirable to the malting industry and results in significant economic loss annually. To identify proteins associated with barley black point we utilised a proteomic approach with 2-DE to compare proteins from whole grain samples of black pointed and healthy gr...

Anhydrobiotic animals protect cellular architecture and metabolic machinery in the dry state, yet the molecular repertoire supporting this profound dehydration tolerance is not fully understood. For the desiccation-tolerant crustacean, Artemia franciscana, we report differential expression of two distinct mRNAs encoding for proteins that share sequence similarities and structural features with ...

The Ciidae genus Anoplocis was established by Makoto Kawanabe (1996) based on the type species Ennearthron poriae Nakane & Nobuki, 1955 from Japan. Additionally, he described the Japanese species Anoplocis ryukyuensis Kawanabe, 1996. However, the name Anoplocis was previously proposed by Arthur M. Lea (1913) for a genus of true weevils (Coleoptera: Curculionidae), with its type species Anoploci...

Lord Howe is an oceanic and relatively young island situated in an area of complex geological and therefore biogeographical processes. The island boasts a large number of endemic species, including many beetles, however, few groups are in an adequate state of systematic knowledge for biogeographic investigation. Recent advances in the systematics of the hyper-diverse rove beetle tribe Staphylin...

Islet transplantation is a potential treatment for type 1 diabetes, but the shortage of donor organs limits its routine application. As potential donor animals, we generated transgenic pigs expressing LEA29Y, a high-affinity variant of the T-cell costimulation inhibitor CTLA-4Ig, under the control of the porcine insulin gene promoter. Neonatal islet cell clusters (ICCs) from INSLEA29Y transgeni...

In WISA’13, a novel lightweight block cipher named LEA was released. This algorithm has certain useful features for hardware and software implementations, i.e., simple ARX operations, non-S-box architecture, and 32-bit word size. These features are realized in several platforms for practical usage with high performance and low overheads. In this paper, we further improve 128-, 192and 256-bit LE...