8 Improved understanding of the biology of reproduction in filarial worms may lead to identification of new targets for drugs or vaccines. Real-time RT-PCR is increasingly being adopted for RNA quantification and genetic analysis. Candidate gender-regulated genes were selected from genes identified in prior studies by differential display RT-PCR and by electronic selection of the Brugia malayi ...

اهتفاقثو اهرخفو ةملأا ةيأ ةيوه نم أزجتي لا اءزج يرامعملا ثارتلا لكشي . ةيخيراتلا ةرامعلا رصانع نا يف اهؤانبو اهميمصت مت دق ةيملاسلإا تابلطتمل يويحو لاعف لكشب ةباجتسلاا ىلع ةرداق نوكت ثيحب يضاملا ةينيدلاو ةيسفنلاو ةيعامتجلااو ةيئيبلاو ةيداملا سانلا . رضاحلا تقولا يف نويرامعملا نوسدنهملا موقي رصانعلا ذخأب اوذخأي ام ةداعو ميمصتلا عيراشم يف اهلاخدا و ةيملاسلإا ةيخيراتلا ينابملا نم ةفلتخملا نيوكت ر...

Human rhinoviruses (HRVs) are positive-stranded RNA viruses belonging to the Enterovirus genus in the family of Picornaviridae. Identification of the specific strain in HRV disease has been difficult because the traditional serological method is insensitive, labor intensive, and cumbersome. With the fast progress in molecular biological technique, more sensitive and faster molecular methods hav...

Peste des petits ruminants (PPR) is a viral disease of small that highly contagious, severe, reportable, and economically important. The present study was conducted to detect the PPR virus (PPRV) circulating in sheep Bangladesh determine its association with epidemiological risk factors degree relationship between F H genes PPRV those other goat isolates. A cross-sectional five selected distric...

The use of reverse transcription (RT) PCR for relative quantitation of gene transcripts relies on the reproducibility of the individual RT, PCR and product measurement steps. Semi-competitive RT-PCR (RT-cPCR) uses an internal competitor template in the PCR step to improve quantitation. We have surveyed the reproducibility of RT, PCR, RT-cPCR and measurement, amplifying the glyceraldehyde-3-phos...

Abstract Transgenic plants expressing immunoglobulin (Ig) M Fc-fused Prostate acid phosphatase (PAP) antigenic proteins (PAP-IgM FcK) and J-chain were generated by Agrobacterium-mediated transformation. The Fc region was tagged with the ER retention motif (KDEL) to make PAP-IgM FcK. Two transgenic crossed together generate F 1 both FcK × J-chain). PCR RT-PCR analyses confirmed transgene inserti...