نتایج جستجو برای: پروتئین نوترکیب tgf β1

تعداد نتایج: 52939  

Journal: :Acta cirurgica brasileira 2014
Antonio Carlos Aloise Max Domingues Pereira Silvio Eduardo Duailibi Alfredo Gragnani Lydia Masako Ferreira

PURPOSE To evaluate the role of transforming growth factor beta 1 (TGF-β1) on the induced osteogenic differentiation of human dermal fibroblasts. METHODS We performed four groups with cultured dermal fibroblasts according to the culture medium: CONTROL (DMEM culture medium); TGF-β1 (DMEM culture medium with 10 ng/ml of TGF-β1); OSTEOG (DMEM culture medium with 0.5 µg/ml of ascorbic acid, 10 m...

2014
Reiko Kimura-Tsuchiya Takeshi Ishikawa Satoshi Kokura Katsura Mizushima Satoko Adachi Manabu Okajima Tatsuzo Matsuyama Tetsuya Okayama Naoyuki Sakamoto Kazuhiro Katada Kazuhiro Kamada Kazuhiko Uchiyama Osamu Handa Tomohisa Takagi Nobuaki Yagi Yuji Naito Yoshito Itoh

Epithelial-mesenchymal transition (EMT) plays a crucial role in cancer metastasis. In this study, we evaluated the effect of heat treatment on tumor growth factor-β1 (TGF-β1)-induced EMT in pancreatic cancer cells and tried to ascertain the mechanism related to any observed effects. Human pancreatic cancer cell lines (BxPC-3, PANC-1 and MIAPaCa-2) were stimulated by TGF-β1, and evaluated for mo...

Journal: :Advances in Clinical and Experimental Medicine 2021

Background Wound healing is an essential physiological process in recovery after microsurgery. Objectives To further understand the functions of fibroblast growth factor 21 (FGF21), roles this were examined and its correlations with inflammation, vascular endothelial A (VEGFA) ERK1/2 signaling pathway activation analyzed. Material methods Rat brain microvascular cells (RBMECs) treated interleuk...

2012
Jasvir Kaur Dieter P. Reinhardt

Extracellular recombinant proteins are commonly produced using HEK293 cells as histidine-tagged proteins facilitating purification by immobilized metal affinity chromatography (IMAC). Based on gel analyses, this one-step purification typically produces proteins of high purity. Here, we analyzed the presence of TGF-β1 in such IMAC purifications using recombinant extracellular fibrillin-1 fragmen...

2014
Abeer Mohy Ahmed Fouad

OBJECTIVES Liver cirrhosis is a condition in which the liver slowly deteriorates and malfunctions due to chronic injury. HCV is one of the major causes of liver fibrosis and ultimate progression to cirrhosis. Transforming growth factor-beta1 (TGF-β1), one of the three isoforms of TGF-β, is a pleiotrophic cytokine that regulates the proliferation and differentiation of cells, embryonic developme...

Journal: :iranian journal of immunology 0
sara assadiasl department of immunology, school of medicine pedram ahmadpoor chronic kidney disease research center, labbafinejad hospital, shahid beheshti university of medical sciences mohsen nafar chronic kidney disease research center, labbafinejad hospital, shahid beheshti university of medical sciences mahboob lessan pezeshki nephrology research center, tehran university of medical sciences fateme pourrezagholi chronic kidney disease research center, labbafinejad hospital, shahid beheshti university of medical sciences mahmoud parvin department of pathology, labbafinejad hospital, shahid beheshti university of medical sciences, tehran, iran abtin shahlaee

background: regulatory t cells have been suggested to have a protective role against acute rejection in allograft recipients. however, there is little information available about their contribution to chronic rejection process. the role of transforming growth factor-beta 1 (tgf- β1) as a profibrogenic and/or immunoregulatory cytokine in renal allografts is also controversial. objectives: to eva...

2014
Chang-Sook Hong Laurent Muller Theresa L. Whiteside Michael Boyiadzis

PURPOSE Exosomes isolated from the plasma of newly diagnosed acute myeloid leukemia (AML) patients have elevated protein and transforming growth factor-beta 1 (TGF-β1) contents and inhibit natural killer (NK) cell cytotoxicity (Haematologica 96, p. 1302, 2011). A potential role of exosomes in predicting responses to chemotherapy (CT) was evaluated in AML patients undergoing treatment. EXPERIM...

ژورنال: :طب جانباز 0
فائزه قانع زاده faezeh ghane zadeh department of biochemistry, science and research branch, islamic azad university, tehran, iranگروه بیوشیمی ،دانشگاه آزاد اسلامی واحد علوم و تحقیقات،تهران،ایران عباسعلی ایمانی فولادی abbas ali imani fooladi applied microbiology research center, baqiyatallah university of medical sciences tehran, iranدانشگاه علوم پزشکی بقیته الله (عج)،مرکز میکروبیو لوژی کاربردی منیره سادات میرزمانی بافقی monnireh sadat mirzaman bafghi chemical injuries research center , baqiyatallah university of medical sciences tehran, iranدانشگاه علوم پزشکی بقیه الله (عج)، مرکز تحقیقات آسیب های شیمیایی مینا میرباقری mina mirbagheri محمد رضا نورانی mohammad reza nourani chemical injuries research center , baqiyatallah university of medical sciences tehran, iranدانشگاه علوم پزشکی بقیه الله (عج)، مرکز تحقیقات آسیب های شیمیایی

زمینه: سولفور موستارد یک عامل تاول زا می باشد که در طی جنگ ایران و عراق به عنوان سلاح جنگی مورد استفاده قرار گرفته است. یکی از مهمترین بافت های درگیر در صدمات ناشی از گاز خردل ریه می باشد. تغییر در ساختار مورفولوژیکی راه های هوایی و فیبروز اطراف برونشیول ها به دنبال استنشاق گاز خردل در جانبازان شیمیایی با عارضه مزمن انسدادی راه های هوایی (copd) دیده می شود.یکی از مولکول هایی که در ایجاد فیبروز ...

1996
Grainne A. MCMAHON John D. DIGNAM Larry E. GENTRY

The formation of a non-covalent complex between mature transforming growth factor β1 (TGF-β1) and its pro region, the β1-latency-associated peptide (β1-LAP), is important in regulating the activity of this multipotent growth factor. We have overexpressed simian β1-LAP in Chinese hamster ovary (CHO) cells to produce a cell line which secretes β1-LAP into the culture medium at " 1 mg}l, thus enab...

2016
Pattranuch Chusri Kattareeya Kumthip Jian Hong Chuanlong Zhu Xiaoqiong Duan Nikolaus Jilg Dahlene N. Fusco Cynthia Brisac Esperance A. Schaefer Dachuan Cai Lee F. Peng Niwat Maneekarn Wenyu Lin Raymond T. Chung

HCV replication disrupts normal endoplasmic reticulum (ER) function and activates a signaling network called the unfolded protein response (UPR). UPR is directed by three ER transmembrane proteins including ATF6, IRE1, and PERK. HCV increases TGF-β1 and oxidative stress, which play important roles in liver fibrogenesis. HCV has been shown to induce TGF-β1 through the generation of reactive oxyg...

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