Microarrays are one of the new emerging methods in plant virology currently being developed by various laboratories. In this study, a new approach is described on the detection of plant viruses using short synthetic single-stranded oligomers (40 nt) instead of PCR products as capture probes. A microchip detecting potato viruses, PVA, PVS, PVM, PVX, PVY and PLRV, in both single and mixed infecti...

To counteract host antiviral RNA silencing, plant viruses express suppressor proteins that function as pathogenicity enhancers. The genome of the Tomato chlorosis virus (ToCV) (genus Crinivirus, family Closteroviridae) encodes an RNA silencing suppressor, the protein p22, that has been described as having one of the longest lasting local suppressor activities when assayed in Nicotiana benthamia...

Abstract Testing for potato viruses is globally very important to prevent a critical shortage of supply. In most countries, testing obligated by law. Germany, seed potatoes are monitored six viruses: PLRV, PVY, PVM, PVA, PVX and PVS. They can cause up 90% loss tubers in the field. Common methods currently used ELISA conventional real-time PCR, but both time-consuming, former needs high capacity...

Abstract Potato viruses PLRV, PVY, PVM, PVA, PVX and PVS can cause up to 90% loss of potato harvest. Therefore, they are monitored by law in many countries using DAS-ELISA or conventional real-time RT-qPCR. Previously, we developed a multiplex DiRT-PCR (Direct reverse transcript – polymerase chain reaction), which works directly on diluted tuber sap thus saves time chemical processing for RNA e...