We have investigated human lactate dehydrogenase (LDH) isoenzymes and human nuclear matrix protein 41/7 (NMP 41/7) as potential serologic markers to monitor the course of human leukemia in severe combined immunodeficient (SCID) mice. Following the transplantation of 10(6) human acute lymphoblastic leukemia (ALL) Nalm-6 cells, human specific LDH isoenzymes were measurable in the serum of SCID mi...

BACKGROUND Application of follicular fluid (FF) and platelet-activating factor (PAF) in artificial insemination improves sperm motility. Lactate dehydrogenase C (LDH-C) is a key enzyme for sperm motility. In this study, the effects of FF and PAF on the sperm motility index and LDH-C expression were investigated. Moreover, LDH-C expression was compared between asthenozoospermic and normozoosperm...

The heterogeneity of lactic dehydrogenase (LDH) activity in human serum and erythrocytes has been observed in several laboratories (1-7). If each of the electrophoretically-distinguishable enzymes in serum with LDH activity were derived from different tissues, in disease states involving a single organ the normal pattern of serum LDH activity would be altered according to the type of LDH releas...

Erythrocytes from representatives of the 5 classes of vertebrates revealed a marked species variation in the number of LDH isozymes, in the distribution of the total LDH activity among these isozymes, and in their electrophoretic mobilities. Starch gel electrophoresis of hemolysates followed by direct histochemical demonstration of LDH activity with nitro blue tetrazolium as dye and phenazine m...

Diagnosis of subclinical mastitis is of increasing importance and appropriate detection methods are needed. Both haptoglobin (Hp), an acute phase protein in cattle, as well as lactate dehydrogenase (LDH), an ubiquitous enzyme, can be successfully used to detect clinical mastitis. The present paper describes quantification of Hp and LDH in milk samples from healthy and subclinically diseased udd...

Sperm specific lactate dehydrogenases (LDH-C₄) is a lactate dehydrogenase that catalyzes the conversion of pyruvate to lactate. In mammals, Ldh-c was originally thought to be expressed only in testes and spermatozoa. Plateau pika (Ochotona curzoniae), which belongs to the genus Ochotona of the Ochotonidea family, is a hypoxia-tolerant mammal living 3000-5000 m above sea level on the Qinghai-Tib...

Serum lactate dehydrogenase (LDH) concentrations correlate with tumor progression and poor outcome. We evaluated the predictive value of serum LDH level for overall survival (OS) of patients with advanced pancreatic cancer after gemcitabine-based chemotherapy. We retrospectively enrolled 364 patients with locally advanced or metastatic pancreatic adenocarcinoma who were then allocated to traini...

Serum LDH levels have been found to be significantly increased in non-Hodgkin lymphoma (NHL) patients, both histiocytic and lymphocytic. The duration of survival of NHL negatively correlates with the level of serum lactic dehydrogenase (LDH), and statistical analysis reveals that patients with lower levels of LDH have a longer survival rate than the patients with higher LDH activity, irrespecti...

Human platelets and bovine lens fibers contain lactate dehydrogenase-3 (LDH-3) as the predominant isozyme but only very faint traces of LDH-5. Since the platelets and lens fibers, like mature human erythrocytes, lack a nucleus, the results strengthen the case for a previously developed association between LDH-5 and the cell nucleus. These three cell types are mainly anaerobic, and therefore the...