Prostate cancer stem-like cells (PCSLCs) are considered to be the 'seed' of prostate cancer. The aim of this study was to confirm that the PC-3 cells, which we isolated and enriched from PC-3 cells through magnetic bead cell sorting (MACS) and serum-free medium (SFM) culture, were PCSLCs. Combinations of MACS, flow cytometry (FCM), SFM and immunocytochemistry (ICC) were used to ensure the posit...

OBJECTIVE The existence of female germ-line stem cells (FGSCs) has been the subject of a wide range of recent studies. Successful isolation and culture of FGSCs could facilitate studies on regenerative medicine and infertility treatments in the near future. Our aim in the present study was evaluation of the most commonly used techniques in enrichment of FGSCs and in establishment of the best pr...

We describe the design and current status of a new X-ray cluster survey aimed at the compilation of a statistically complete sample of very X-ray luminous (and thus, by inference, massive), distant clusters of galaxies. The primary goal of the MAssive Cluster Survey (MACS) is to increase the number of known massive clusters at z > 0.3 from a handful to hundreds. Upon completion of the survey, t...

BACKGROUND Externalization of phosphatidylserine (EPS) occurs in apoptotic-like spermatozoa and could be used to remove them from sperm preparations to enhance sperm quality for assisted medical procreation. We first characterized EPS in sperms from infertile patients in terms of frequency of EPS spermatozoa as well as localization of phosphatidylserine (PS) on spermatozoa. Subsequently, we det...

Fetal nucleated cells in the maternal circulation constitute a potential source of cells for the non-invasive prenatal diagnosis of fetal genetic abnormalities. We have investigated the use of the Magnetic Activated Cell Sorter (MACS) for enriching fetal nucleated erythrocytes. Mouse monoclonal antibodies specific for CD45 and CD32 were used to deplete leucocytes from maternal blood using MACS ...

We present results of a strong-lensing analysis of MACS J0717.5+3745 (hereafter MACS J0717), an extremely X-ray luminous galaxy cluster at z = 0.55. Observations at different wavelengths reveal a complex and dynamically very active cluster, whose core is connected to a large scale filament extended over several Mpc. Using multi-passband imaging data obtained with the Hubble Space Telescope’s Ad...