The role of cell surface glycoproteins in determining in vivo blood-borne arrest and survival characteristics of murine melanoma sublines of low (B16-F1) or high (B16-F10) potential to form experimental lung metastases after injection i.v. was assessed after inhibiting tumor cell protein glycosylation with tunicamycin. Incubation of B16-F1 or B16-F10 cells with 0.5 micrograms (or above) tunicam...

PURPOSE The most aggressive form of skin cancer is the malignant melanoma. Because of its high metastatic potential the early detection of primary melanoma tumors and metastases using non-invasive PET imaging determines the outcome of the disease. Previous studies have already shown that benzamide derivatives, such as procainamide (PCA) specifically bind to melanin pigment. The aim of this stud...

PURPOSE Tumor cell metastasis constitutes a major problem in the treatment of cancer. Because the cure rate of metastatic tumors is very low, new therapeutic approaches are needed. Heat shock protein 90 (HSP90) is a molecular chaperone that is recognized as a new target for the treatment of cancer. Here, we examine the value of a monoclonal antibody (mAb) against HSP90, mAb 4C5, as a potential ...

Breast cancer most frequently metastasizes to bone causing decreased quality of life and morbidity. Since current treatments are palliative, strategies to prevent bone metastases in breast cancer patients are required. There is substantial evidence indicating that high levels of nitric oxide (NO) suppress tumor growth and metastasis in vivo. We hypothesize that agents that produce high concentr...

به منظور ارتقای سطح کیفی فضاهای مسکونی، در این پژوهش هدف کشف عوامل مؤثر بر کیفیت محیط ارتباط بین آن‌ها و بیان راهبردهایی همین است. مسکونی مفهومی پویا، پیچیده، چندبعدی است که از ابعاد مختلف کالبدی، اجتماعی، اقتصادی، زیست‌‌محیطی شکل گرفته معیارهای مناسب ارزیابی سنجش رضایتمندی ساکنان آن ماهیت اما دلیل استخراج قابل‌اندازه‌‌گیری تلفیقی روش‌‌های کمّی قالب شیوۀ همبستگی استفاده شده با تبیین الگوی معادله...

BACKGROUND The 16 kDa heat shock protein (HSP) is an immuno-dominant antigen, used in diagnosis of infectious Mycobacterium tuberculosis (M.tb.) causing tuberculosis (TB). Its use in serum-based diagnostics is limited, but for the direct identification of M.tb. bacteria in sputum or cultures it may represent a useful tool. Recently, a broad set of twelve 16 kDa specific heavy chain llama antibo...

F10, the gene encoding the major eggshell protein of the parasitic trematode Schistosoma mansoni has been recently cloned and sequenced (1, 2). The nucleotide sequence predicted a protein with 160 amino acids and a 48.7% content of glycine. Most of the glycines are in a stretch extending from amino acid 25 to 112, corresponding to nucleotides 329 to 593 in the gene sequence. We aligned this str...

Hypoxia-inducible factor-1 (HIF-1) governs cellular adaption to the hypoxic microenvironment and is associated with a proliferative, metastatic, and treatment-resistant tumor phenotype. HIF-1 levels and transcriptional activity are regulated by proline and asparagine hydroxylases, which require ascorbate as cofactor. Ascorbate supplementation reduced HIF-1 activation in vitro, but only limited ...

Angioarrestin is a recently described anti-angiogenic protein whose expression is down-regulated in solid tumours of various origins. It has a sequence identical to angiopoietin related protein-1. In this study we investigated anti-tumour properties of angioarrestin in B16 (F10) melanoma tumour model. We constructed an expression vector encoding human angioarrestin under the control of EF-1alph...