نتایج جستجو برای: مارکرهای مولکولی rapd

تعداد نتایج: 17392  

Journal: :Journal of clinical microbiology 1999
H Vu-Thien C Dulot D Moissenet B Fauroux A Garbarg-Chenon

Randomly amplified polymorphic DNA (RAPD) and pulsed-field gel electrophoresis (PFGE) for the analysis of 13 Moraxella catarrhalis isolates, 11 successive strains isolated from sputa of five children and 2 isolates obtained the same day from twins, were compared. RAPD and PFGE both yielded nine types from the 13 isolates, showing a chronic colonization with one strain in three patients and a su...

Journal: :Journal of clinical microbiology 1993
B G Corney J Colley S P Djordjevic R Whittington G C Graham

We compared random amplified polymorphic DNA (RAPD) fingerprinting with cross-absorption agglutination and restriction enzyme analysis for typing bovine leptospires. Using RAPD fingerprinting, we examined a number of Leptospira serovars, namely, hardjo genotypes bovis and prajitno, pomona, balcanica, tarassovi, swajizak, kremastos, australis, and zanoni, which are likely to be isolated from Aus...

2013
Mitra Ashayeri-Panah Fereshteh Eftekhar Maryam Mobarak Ghamsari Mahmood Parvin Mohammad Mehdi Feizabadi

In this study, the discriminatory power of pulsed field gel electrophoresis (PFGE) and random amplified polymorphic DNA (RAPD) methods for subtyping of 54 clinical isolates of Klebsiella pneumoniae were compared. All isolates were typeable by RAPD, while 3.6% of them were not typeable by PFGE. The repeatability of both typing methods were 100% with satisfying reproducibility (≥ 95%). Although t...

2016
Carolina P. B. Gracitelli Andrew J. Tatham Linda M. Zangwill Robert N. Weinreb Ricardo Y. Abe Alberto Diniz-Filho Augusto Paranhos Saif Baig Felipe A. Medeiros

PURPOSE We examined the relationship between relative afferent pupillary defects (RAPDs) and macular structural damage measured by macular thickness and macular ganglion cell-inner plexiform layer (mGCIPL) thickness in patients with glaucoma. METHODS A cross-sectional study was done of 106 glaucoma patients and 85 healthy individuals from the Diagnostic Innovations in Glaucoma Study. All subj...

2017
Aejaz Ahmad Dar Sushma Mudigunda Pramod Kumar Mittal Neelakantan Arumugam

Sesame (Sesamum indicum L.) is an ancient oilseed crop known for its nutty seeds and high-quality edible oil. It is an unexplored crop with a great economic potential. The present study deals with assessment of genetic diversity in the crop. Twenty two RAPD and 18 SSR primers were used for analysis of the 47 different sesame accessions grown in different agroclimatic zones of India. A total of ...

Journal: :Journal of hazardous materials 2009
Wan Liu Y S Yang P J Li Q X Zhou L J Xie Y P Han

Impact assessment of contaminants in soil is an important issue in environmental quality study and remediation of contaminated land. A random amplified polymorphic DNA (RAPD) 'fingerprinting' technique was exhibited to detect genotoxin-induced DNA damage of plants from heavy metal contaminated soil. This study compared the effects occurring at molecular and population levels in barley seedlings...

2005
G. OZBEY H. B. ERTAS A. MUZ

Six field strains of Ornithobacterium rhinotracheale isolated from chickens in Elazig province located in the East of Turkey were typed by serotyping and random amplified polymorphic DNA assay using a random primer (OPG-11). Using the AGP test used for serotyping, serotype A was found to be the predominant serotype, only one strain was serotyped as serotype B. By RAPD assay, the tested ORT stra...

Journal: :Memorias do Instituto Oswaldo Cruz 2008
Cintia de Moraes Borba Janaina Correia Ester Vinhas Albert Martins Beatriz Costa Aguiar Alves Sheila Unkles James Robertson Kinghorn Norma Lucena-Silva

Molecular characterization of Paracoccidioides brasiliensis variant strains that had been preserved under mineral oil for decades was carried out by random amplified polymorphic DNA analysis (RAPD). On P. brasiliensis variants in the transitional phase and strains with typical morphology, RAPD produced reproducible polymorphic amplification products that differentiated them. A dendrogram based ...

2014
Z. Gálová M. Wójcik

In the present study, RAPD-PCR was used to assess genetic diversity of the rye including landrances and new rye cultivars coming from Central Europe and the Union of Soviet Socialist Republics (SUN). Five arbitrary random primers were used to determine RAPD polymorphism in the set of 38 rye genotypes. These primers amplified altogether 43 different DNA fragments with an average number of 8.6 fr...

Journal: :Journal of optometry 2018
Eulogio Besada Barry J Frauens Rim Makhlouf Diana Shechtman Julie Rodman Marlon Demeritt Patrick Hardigan

PURPOSE This study investigated the correlation between the relative afferent pupillary defect (RAPD) and retinal nerve fiber layer thickness (RNFLT) in optic neuropathy. METHODS RAPD assessment was performed using a log unit neutral density filter bar. Spectral domain optical coherence tomography RTVue-100 (Optovue) was used to examine the subjects. The optic nerve head pattern (ONH) was sub...

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