OBJECTIVES This work deals with the conformational and thermal characterization of a synthetic peptide (S4) released during the proteolysis of human tropoelastin by the matrix metalloproteinase-12 that was shown to form amyloid-like fibres under certain conditions. MATERIALS AND METHODS S4 peptides were synthesized by solid-phase methodology and aggregated in solution at 80°C. Fourier transfo...

The aim of the present study was to investigate the possibility of using infrared spectroscopy for detecting the irradiation of meats. Samples of beef meat containing fat were subjected to gamma irradiation at doses of 0, 1.5, 3 and 4.5 kGy at room temperature. Then irradiated and non-irradiated beef meat samples were examined by Fouriertransform infrared (FT-IR) spectroscopy post irradiation t...

Fourier transform infrared (FT-IR) spectroscopy combined with multivariate statistical analyses was investigated as a physicochemical tool for monitoring secreted recombinant antibody production in cultures of Chinese hamster ovary (CHO) and murine myeloma non-secreting 0 (NS0) cell lines. Medium samples were taken during culture of CHO and NS0 cells lines, which included both antibody-producin...

Purpose To compare the results of a chemical method of kidney stone analysis with the results of Fourier transform infrared (FT-IR) spectroscopy. Materials and Methods Kidney stones collected between June and October 2015 were simultaneously analyzed by chemical and FT-IR methods. Results Kidney stones (n=449) were collected from patients from 1 to 81 years old. Most stones were from adults...

Through the use of site-directed mutagenesis and chemical rescue, we have identified the proton acceptor for redox-active tyrosine D in photosystem II (PSII). Effects of chemical rescue on the tyrosyl radical were monitored by EPR spectroscopy. We also have acquired the Fourier-transform infrared (FT-IR) spectrum associated with the oxidation of tyrosine D and concomitant protonation of the acc...

Fourier transformation infrared (FT-IR) spectroscopy has been used to measure glucose concentrations in different matrices. The accuracy of the FT-IR technique does not meet the requirements of medical applications, so we have developed a new, efficient and precise method based on attenuated total reflectance coupled with wavelet transformation (ATR-WT-IR). One thousand interferograms, divided ...

Horizontal attenuation total reflection Fourier transformation infrared spectroscopy (HATR-FT-IR) studies on cuscutae semen and its confusable varieties Japanese dodder and sinapis semen combined with discrete wavelet transformation (DWT) and radial basis function (RBF) neural networks have been conducted in order to classify them. DWT is used to decompose the FT-IRs of cuscutae semen, Japanese...