هدف انتخاب تأمین‌کنندگان و تخصیص سفارش بر اساس ابعاد پایداری ریسک پایداری، در شرایط عدم‌­قطعیت برخی پارامترها است؛ همچنین با محدودیت استراتژی کاهش برای مدیریت همراه است. معیارهای ریسک، امتیاز توسط روش‌های تاپسیس فازی تجزیه‌وتحلیل آثار شکست محاسبه شده است سپس برنامه تصادفی چندمرحله‌ای ایجاد معیار ارزش معرض شرطی، پیرامون تأمین منابع یک فضای چند­دوره‌ای برنامه‌‎ریزی دستیابی به برنامه‌ریزی انعطاف‌پ...

A total of 1120 specimens of fresh urine from male patients was tested for Chlamydia trachomatis with the Roche Amplicor PCR Kit and an in-house PCR assay. The in-house PCR had an internal control to monitor inhibitory effects of clinical specimens on the PCR assay. All urine samples were processed within 24-48 h of collection and DNA was extracted on the same day that the assays were performed...

The prevalence of microsporidiosis is likely underestimated due to the labor-intensive, insensitive, and nonspecific clinical laboratory methods used for the diagnosis of this disease. A real-time PCR assay was designed to assess DNA extraction methods and to detect three Encephalitozoon species in feces. Modifications of the MagNA Pure LC DNA isolation kit protocol (Roche Applied Sciences, Ind...

Though HCV infection is a serious public health problem, some aspects of its biology are still not well understood, such as its transmission through seminal fluid and sexual transmission. We looked for HCV in the semen of infected patients. Thirteen patients were included. Semen fractions (seminal plasma, leukocytes and spermatozoa) were separated with 45% and 90% Percoll gradients. The HCV-RNA...

STUDY OBJECTIVES In adults, arterial blood samples are usually drawn using plastic syringes. In contrast to glass syringes, plastic syringes let oxygen diffuse through their wall. This results in PO(2) changes during storage, especially when PO(2) is high. An alternative to glass syringes is the Microsampler (Roche Diagnostics; Schaffhausen, Switzerland), a commercially available device consist...