Excessive zinc influx may contribute to neuronal death after certain insults, including transient global ischemia. In light of evidence that levels of intracellular free Zn(2+) associated with neurotoxicity may be sufficient to inhibit glyceraldehyde-3-phosphate dehydrogenase (GAPDH), experiments were performed looking for reduced glycolysis and energy failure in cultured mouse cortical neurons...

On subcellular fractionation, the enzyme acyl/alkyl dihydroxyacetone phosphate (DHAP) reductase (EC 1.1.1.101) in guinea pig and rat liver was found to be present in both the light mitochondrial (L) and microsomal fractions. By using metrizamide density gradient centrifugation, it was shown that the alkyl DHAP reductase activity in the "L" fraction is localized mainly in peroxisomes. From the d...

The photosynthetic cyanobacterium Synechocystis sp. strain PCC6803 possesses homologs of known genes of the non-mevalonate 2-C-methyl-D-erythritol 2-phosphate (MEP) pathway for synthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Isoprenoid biosynthesis in extracts of this cyanobacterium, measured by incorporation of radiolabeled IPP, was not stimulated by pyruvate,...

Figure 1. Scheme of a kinetic model of central intracellular metabolism. Red arrows indicate glycolysis, magenta pentose-phosphate pathway, green gluconeogenesis, and blue tricaboxylic acid (TCA) cycle. Black arrows indicate exchanges with metabolites, external with respect to the model variables: glc (glucose), g6p (glucose 6-phosphate), f6p (fructose 6phosphate), fbp (fructose phosphate), g3p...

Anaplastic large cell lymphoma (ALCL) is a common subtype of the heterogeneous group of peripheral T-cell lymphomas, which is characterized by large pleomorphic cells with strong expression of CD30. Translocations involving ALK, the anaplastic lymphoma kinase gene, are associated with a favorable clinical outcome. Such ALK-positive ALCLs are usually responsive to a multidrug chemotherapy with C...

The enzymes for the biosynthesis of phosphatidic acid from acyl dihydroxyacetone phosphate were shown to be present in rat brain. These enzymes were mainly localized in the microsomal fraction of 12--14 day old rat brains. The brain microsomal acyl C0A:dihydroxyacetone phosphate acyl transferase (EC 2.3.1.42). exhibited a broad pH optimum between pH 5 and 9 with maximum activity at pH 5.4. K, f...

DeMarc A. Hickson1,2,3, Lance A. Waller1, Lillian S. Lin3 1Emory University, Rollins School of Public Health, Department of Biostatistics 1518 Clifton Road, Atlanta, Georgia, 30322, U.S.A. E −mail : [email protected]; Telephone: 404.727.1057; Fax: 404.727.1370 2Northrop Grumman Mission Systems, 12011 Sunset Hills Road, Reston, Virginia 20190, U.S.A. 3Centers for Disease Control and Preventi...

An infant with the characteristic phenotype of classical rhizomelic chondrodysplasia punctata was found to have an isolated deficiency of the peroxisomal enzyme acyl CoA dihydroxyacetone phosphate acyltransferase (DHAP-AT). All other peroxisomal functions measured were found to be normal. Previously described in one other case report, this confirms the existence of another distinct form of pero...