AIM To conduct developmental validation studies on a polymerase chain reaction (PCR) based short tandem repeat (STR) multiplex typing system, developed for the purpose of genetic individualization and parentage testing in domestic cat samples. METHODS To evaluate reproducibility of the typing system, the multiplex was amplified using DNA extracted from hair, blood, and buccal samples obtained...

BACKGROUND At least six HCV (hepatitis C virus) genotypes are unequally distributed worldwide. HCV genotyping guides the selection of treatment regimens and provides important epidemiological markers that enable the outbreak source to be traced and the spread of disease to be controlled. In Egypt, there is an increasing need for cost-effective, fast, and easily performable HCV genotyping assays...

Researchers carrying out quantitative, multiplex, realtime PCR and RT-PCR require master mixes that provide accurate, simultaneous quantification of multiple targets in the same reaction without time-consuming optimization. Our results at Wyeth Research show that QuantiTect® Multiplex Kits from QIAGEN are well suited for multiplex analysis of up to four RNA targets in two-step and onestep real-...

shigella species are among the common causes of bacterial diarrhoeal diseases. traditional detection methods are time-consuming resulting in delay in treatment and control of shigella infections thus there is a need to develop molecular methods for rapid and simultaneous detection of shigella spp. in this study a rapid multiplex pcr were developed for simultaneous detection of three pathogenic ...

introduction: awareness of the species of shigella has particular importance in the way of dealing with an outbreak, controlling it, and treating patients. the purpose of this study was to use the multiplex pcr method and comparison with the culture method to detect shigella species, as well as analysing the antibiotic resistance pattern in its different species. methods: simultaneously, the fe...

the present study was designed to investigate the prevalence of salmonella species, their molecular characterization and antibiotic resistance in backyard chickens. a total of 1125 samples were collected from backyard chickens in two consequent samplings. in the first part, samples were included of 820 poor recently hatched chicks, hatching residuals, egg shells in the nest floor, cloacal swabs...

background and objectives: pcr is a sensitive assay and could be used as an accurate diagnostic method for detecting various types of microorganisms' genome in low concentration in biological specimens. the demand for sensitive, rapid, safe and easy detection of pcr products has led researchers to a combination of this method with elisa. materials and methods: conserved sequences were selected ...

Epstein-Barr virus (EBV), a human herpesvirus, affects up to 95% of adults. Diagnosis of acute EBV infection can be challenging and often relies on the serologic antibody pattern to 3 distinct antigens, most often determined by indirect fluorescent antibody (IFA), enzyme-linked immunosorbent assays (ELISAs), and, more recently, multiplex assays. We compared a multiplex assay for the simultaneou...