Aiming at the exploration of herbal use by society, crude extracts of the seeds of some commonly used medicinal plants (Vitis vinifera, Tamarindus indica and Glycin max) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. The overall antioxi...

The objective of the study was to investigate the in vitro antioxidation activity of lycium barbarum polysaccharides (LBP). Ultraviolet spectrophotometry was adopted to determine the capability of LBP to clear superoxide anions, hydroxyl radicals, DPPH free radicals and ABTS free radicals. The result showed that the law for LBP to clear superoxide anions, hydroxyl radicals and DPPH free radical...

Eugenol, isoeugenol, caffeic acid, ferulic acid, isoferulic acid, estragole, trans-anethole, and paeonol are components of a Chinese herbal medicine used as a painkiller and stomachic. We investigated the potential role of these compounds as antioxidants. We studied the DPPH (1,1-diphenyl-2-picrylhydrazyl) radical-scavenging effect of these molecules, together with some glycoside derivatives, t...

A new flavone C-glycoside, isovitexin 6''-O-E-p-coumarate (1) and two known flavonoid glycosides--quercetin 3-O-beta-D-glucuronopyranoside (2) and isoorientin (3)--were isolated from an ethanol extract of aerial parts of Clematis rehderiana. Their structures were determined by spectroscopic methods. The antioxidant effects of the two flavone C-glycosides were evaluated by both the MTT and DPPH ...

In the present study experiments were carried out to determine the total phenolic content and their comparative antioxidant activities in relation to phenol content employing Folin-ciocalteu, DPPH, and ABTS assays of the different solvent extracts of the plant S. macrosperma. It was found that on an average, the plant extract with higher levels of phenol content had higher antioxidant activity....

The antioxidant potential of the 99% methanolic extract of leaves of Muntingia calabura was assessed by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) and total phenolic content was measured by Folin-Ciocalteau (FC) by Singleton and Rossi using Gallic acid and Tannic acid as the calibration standard. Moreover Muntingia calabura leaf extract showed strong reducing power and significant antioxidant a...

Column chromatography of the Egyptian date palm pollen, resulted in the isolation of estradiol, estrone among ten compounds from the hexane fraction. Also, besides rutin, additional four flavonoids from the ethyl acetate extract were isolated. HPLC analysis of the hexane fraction (using RFBDS C18 – isocratic acetonitrile /water 58:42) revealed the presence of estrone, estradiol and estriol comp...

Phytochemicals profiling is important for the isolation of new and novel chemical substances. The current finding is directed to the detection of different classes of chemical constituents responsible for antioxidant properties of Euphorbia prostrate.The crude methanolic extract and various solvent isolated fractions of E. prostrate showed the presence of Alkaloids, Terpenoids, Saponins, Tannin...

MARINOVA, G. and V. BATCHVAROV, 2011. Evaluation of the methods for determination of the free radical scavenging activity by DPPH. Bulg. J. Agric. Sci., 17: 11-24 The survey of the methods for determination of free radical scavenging activity by DPPH has been done. The differences between methods conditions and their evaluation are presented. It was determined the effect of methods conditions b...

extracts of cotoneaster medicus, glycyrrhiza glabra, as endemic plants of iran, along with mixture of them were investigated for their antioxidant activities using 2, 2- diphenyl-1-picrylhidrazyl (dpph) reagent. uv-vis spectrophotometry method was used to evaluate the ability of cotoneaster and glycyrrhiza glabra antioxidant to scavenge dpph radical. the kinetic parameters such as rate constant...