The release of tumor necrosis factor α (TNF-α) by ochratoxin A (OTA) was studied in various macrophage and non-macrophage cell lines and compared with E. coli lipopolysaccharide (LPS) as a standard TNF-α release agent. Cells were exposed either to 0, 2.5 or 12.5 µmol/L OTA, or to 0.1 µg/mL LPS, for up to 24 h. OTA at 2.5 µmol/L and LPS at 0.1 µg/mL were not toxic to the tested cells as indicate...

It is commonly accepted that the temperature-sensitive phenotype of Sendai virus (SeV) persistently infected cells is caused by the M and/or HN proteins. Expression level of the L, M, HN, and V proteins is extremely low in L929 cells persistently infected with SeVpi (L929/SeVpi cells) incubated at 38 degrees C. The HN protein quickly disappears in L929/SeVpi cells following a temperature shift ...

Cellular phosphorylation events during viral infection are necessary for effective viral replication. Encephalomyocarditis (EMC) virus has been used for studies on the molecular mechanisms of viral replication, but little is known about the cellular signaling pathways involved. This investigation was initiated to determine whether mitogen-activated protein kinases (MAPKs), which are central com...

INTRODUCTION The aims of this study were i) to define the chemical constituents of Cuminum cyminum (cumin) essential oil, ii) to compare the antimicrobial activity of this oil to that of chlorhexidine (CHX) and co-trimoxazole on planktonic and biofilm forms of bacteria isolated from the teeth with persistent endodontic infection and iii ) to compare the cytotoxicity of these medicaments on L929...

Low back pain is one of the most reported medical conditions associated to intervertebral disc (IVD) degeneration. Nucleus pulposus (NP) is often regarded as the structure where IVD degeneration begins. Gellan gum (GG)-based hydrogels for acellular and cellular tissue engineering strategies have been developed for finding applications as NP substitutes. The innovative strategy is based on the r...

هدف از این مطالعه بررسی اثر آب تهی شده دوتریم (DDW) در غلظت­های مشخص بر روی سلول­های 29HT- و 480SW- بود. رده­های سلولی محیط RPMI حاوی مختلف DDW به مدت 24، 48 72 ساعت کشت داده شدند سپس درصد بقای روش سنجش سمیت مبتنی برMTT تعیین شد. یافته­ها نقش توأم کاهش افزایش زمان تیمار را مهار رشد اثبات رساند. کم­ترین میزان برای طی با غلظت ppm 30 ترتیب 23% 37% دست آمد. جهت اثرات جانبی دوتریم، طبیعی 12C2C نیز ت...

When mouse L929 cells are treated with amphotericin B before exposure to polyriboinosinic-polyribocytidylic acid or polyriboinosinic-polyribocytidylic acid-dextran, they make significantly more interferon than do cells not receiving amphotericin. This effect may be due to enhanced cell membrane penetration by the polynucleotide.