Pancreatic beta cell (β) dysfunction is an outcome of malnutrition. We assessed the role of the amplifying pathway (AMP PATH) in β cells in malnourished obese mice. C57Bl-6 mice were fed a control (C) or a low-protein diet (R). The groups were then fed a high-fat diet (CH and RH). AMP PATH contribution to insulin secretion was assessed upon incubating islets with diazoxide and KCl. CH and RH di...

The usefulness of blood enzyme determinations as markers of liver necrosis was tested in 100 alcoholics who underwent biopsy during clinical investigation. Mean values of glutamate dehydrogenase (GDH), serum aspartate and alanine transferase (SGOT and SGPT), ornithine carbamoyltransferase (OCT), and gamma-glutamyltranspeptidase (gamma-GTP) tended to rise with increasing liver cell necrosis, tho...

The assemblages of Giardia duodenalis in 25 children with and 25 children without diarrhoea and 24 adults with gastrointestinal symptoms in South India were determined. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) targeting the glutamate dehydrogenase (gdh), β-giardin and triosephosphate isomerase (tpi) genes was used. The tpi PCR was the most sensitive and dete...

Glutamate dehydrogenase, GDH (L-glutamate:NAD+ oxidoreductase (deaminating) EC 1.4.1.2) was purified from the plant fraction of lupin nodules and the purity of the preparation established by gel electrophoresis and electrofocusing. The purified enzyme existed as 4 charge isozymes with a MW of 270000. The subunit MW, as determined by dodecyl sulphate electrophoresis, was 45000. On the basis of t...

Model-dependence of the single-pion contribution to the GDH, Baldin, and forward spin-polarizability sum rules is explored, using the most recent SAID multipole analysis. Results from SAID and MAID are compared. We have calculated the GDH, Baldin and forward spin-polarizability (γ 0) sum rules using our most recent analysis of single-pion photoproduc-tion data. This set of integrals is useful i...

Brain glutamate dehydrogenase (GDH) activity was compared between streptozotocin induced diabetic rats and insulin treated diabetic rats using crude and purified preparations from rat brain. V. of GDH increased significantly (p<0.05) while Km remained same in diabetic state when compared to control. Insulin treatment restored Vmax to normal values. The observed changes in the present study indi...

A novel enzyme electrode system for glucose oxidation was constructed by co-immobilization of dehydrogenase (GDH) and N,N’-diphenyl-p-phenylenediamine (DPPD) on carbon electrodes. This applied to porous fabric produce a current 7 mA/cm2, while the catalytic activity maintained at approximately 100% more than 4 h, indicating that DPPD molecules were stably retained electrodes during continuous r...

Phenomenological amplitudes obtained in partial-wave analyses (PWA) of single-pion photoproduction are used to evaluate the contribution this process Gerasimov-Drell-Hearn (GDH), Baldin and Gell-Mann-Goldberger-Thirring (GGT) sum rules, by integrating up 2 GeV photon energy. Our study confirms that all these rules converges even before highest considered energy, but levels saturation very diffe...