Escherichia coli was used to investigate quinolone resistance and mutations in gyrA gene of E. coli isolated from pet (dog and cat), human (pet's owner), vegetable and edible ice in Bangkok and vicinity. Susceptibility test for nalidixic acid (NA) showed similar percent resistance among the sample sources but a lower ciprofloxacin (CIP) resistance was found particularly in human source. Mutatio...

INTRODUCTION The aim of this work was to evaluate the prevalence of Mycobacterium tuberculosis (MT) strains with mutations that could result in resistance to the main drugs used in treatment in a region with one of the highest numbers of tuberculosis (TB) cases in southern Brazil. METHODS Deoxyribonucleic acid (DNA) from 120 sputum samples from different patients suspicious of pulmonary tuber...

Hepatitis C virus (HCV) populations in vivo consist of heterogeneous mixtures of genetically different but closely related variants defined as a 'quasispecies'. The longitudinal fluctuation of HCV quasispecies populations in chronic hepatitis C has not been elucidated. Serial plasma samples were obtained from four patients with chronic hepatitis C (two patients without any treatment and two pat...

genotypes of iranian zel sheep for calpastatin(cast) locus were determined by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) and polymerase chain reaction-single strand conformation polymorphism (pcr-sscp) methods and for calpain(capn) locus by pcr-sscp. blood samples were collected from 200 purebred zel sheep of zel breeding station located in golestan province i...

In this study, Substitution at codon Ser315 of katG gene, a reliable marker for isoniazid (INH) resistance was analyzed and compared by three molecular methods such as DNA  sequencing, polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) and PCR-single strand conformation polymorphism (PCR-SSCP) in 105 phenotypically resistant isolates obtained from various parts of Ind...

DOI: 10.1002/sscp.202200019 The cover picture shows that the lead ion (Pb2+) and copper (Cu2+) in lake water are detected by UV-Vis analysis using gold nanoparticles (Au NPs) as colorimetric probe. formation of Pb-O bond between Pb2+ L-Cysteine can induce significant aggregation Au NPs (blue color), conversely, Cu catalyze oxidation Cysteine prevent (pink color). By analyzing absorption spectra...

Single-strand conformation polymorphism (SSCP) analysis is a simple and quick method for mutation detection. It depends on the separation of single-stranded (ss)DNA fragments in non-denaturing polyacrylamide gels. On occasion, the ssDNA bands can be very diffuse or appear against a heavily smeared background. Here, we demonstrate that the addition of denaturants, urea or formamide, to the gels ...