Detection of plasmid DNA uptake in river bacteria at the single-cell level was carried out by rolling-circle amplification (RCA). Uptake of a plasmid containing the green fluorescent protein gene (gfp) by indigenous bacteria from two rivers in Osaka, Japan, was monitored for 506 h using this in situ gene amplification technique with optimized cell permeabilization conditions. Plasmid uptake det...

Rolling circle amplification (RCA) for generation of distinct fluorescent signals in situ relies upon the self-collapsing properties of single-stranded DNA in commonly used RCA-based methods. By introducing a cross-hybridizing DNA oligonucleotide during rolling circle amplification, we demonstrate that the fluorophore-labeled RCA products (RCPs) become smaller. The reduced size of RCPs increase...

Of the remaining 224 publications, 30 were excluded as they were published in a language other than English, ten were excluded as they consisted of reviews or commentaries rather than cases, and an additional 16 publications were excluded as they reported on non-SVG aneurysms, the vein graft was less than 1.5 times the reference vessel, or they represented duplicate reports. One hundred and six...

The endothelial cytoskeleton plays a key role in arterial responses to acute changes in shear stress. We evaluated whether the intermediate filament protein vimentin is involved in the structural responses of arteries to chronic changes in blood flow (BF). In wild-type mice (V+/+) and in vimentin-deficient mice (V-/-), the left common carotid artery (LCA) was ligated near its bifurcation, and 4...

Density and distribution of Unfamiliar Lexical Items (ULIs) appear to influence learners’ Reading Comprehension Achievement (RCA). This study concerns the impact of these two variables on Iranian EFL learners’ RCA. For this, two groups of students timetabled for the experiments designed to assess learners’ RCA. To determine the participants’ levels of proficiency a Quick Proficiency Test was fi...

Rapid developments of time series models and methods addressing volatility in computational finance and econometrics have been recently reported in the financial literature. The non-linear volatility theory either extends and complements existing time series methodology by introducing more general structures or provides an alternative framework (see Abraham and Thavaneswaran [B. Abraham, A. Tha...

In this paper, we investigate and discuss the problem how the abilities of computing and self-reproduction can be realized in a \reversible" environment, especially in reversible cellular automata (RCA). An RCA is a \backward deterministic" CA in which every conngu-ration of the cellular space has at most one predecessor. Such systems have a close connection to physical reversibility, and have ...

A four-bit adder is simulated using HSPICE in two classic design methods: a ripple-carry adder (RCA) and a carry-look-ahead adder (CLA). All components of the adders are composed of PMOS, NMOS, and capacitors. The propagation delay and power dissipation were measured under different VDD values and different operating temperatures in HSPICE. A comparison of these two metrics were analyzed betwee...

A 65-year-old black woman with hypertension and type 2 diabetes mellitus presented to her local hospital with acute chest discomfort. She had no history of connective tissue disease or family history of aortic catastrophes. An ECG showed inferior ST-segment elevation. Coronary angiography revealed multiple high-grade lesions in the right coronary artery (RCA) with evidence of thrombus (Movie I ...

OBJECTIVE Percutaneous coronary intervention for anomalous right coronary artery (RCA) originating from the left coronary cusp is challenging because of our current inability to coaxially engage the guiding catheter. METHODS We report a case of an 88-year-old woman with non-ST segment elevation myocardial infarction, with an anomalous RCA origin. Using either the Judkins-Left catheter or Ampl...