Serial Analysis of Gene Expression (SAGE) is a technique for estimating the gene expression profile of a biological sample. Any efficient inference in SAGE must be based upon efficient estimates of these gene expression profiles, which consist of the estimated relative abundances for each mRNA species present in the sample. The data from SAGE experiments are counts for each observed mRNA specie...

We reviewed the historic and present distribution of Greater Sage-Grouse (Centrocercus urophasianus) in Canada and found that the species has been eliminated from approximately 90% of its estimated historic distribution. Sage-grouse have been extirpated from British Columbia and reduced to remnant populations in Alberta and Saskatchewan. Estimates of the size of the population decline in Canada...

This study evaluated a relatively new molecular technique, serial analysis of gene expression (SAGE), as a tool for quantifying gene expression in the ovine abomasal nematode Haemonchus contortus for which there is relatively limited (approximately 20% gene coverage) sequence information. SAGE technology generates data that are both qualitative and quantitative and, as such, compliments other f...

Conserving migratory ungulates in increasingly human-dominated landscapes presents a difficult challenge to land managers and conservation practitioners. Nevertheless, ungulates may receive ancillary benefits from conservation actions designed to protect species of greater conservation priority where their ranges are sympatric. Greater Sage-Grouse (Centrocerus urophasianus), for example, have b...

BENCHMARKS The serial analysis of gene expression (SAGE) provides high-throughput information on researched samples. SAGE generates 9–13 bp tags from messenger RNA (mRNA) of the sample, and all tags are ligated to form concatemers for cloning and sequencing (1). LongSAGE uses similar procedures but a different restriction enzyme to obtain longer tags (19–21 bp). Both SAGE and LongSAGE tags are ...

Herbal medicines include a range of pharmacologically active compounds: in some cases it is not well understood which ingredients are important for a therapeutic effect. The supporters of herbal medicine believe that isolated ingredients in the majority of cases have weaker clinical effects than whole plant extract, a claim that would obviously require proof in each case. Generalizations about ...

We present the experience of the authors teaching mathematics to freshmen engineering students with the help of the open source computer algebra system Sage. We describe some teaching resources and present an ad hoc distribution of Sage used by the authors.

Greater sage-grouse (Centrocercus urophasianus) are dependent on sagebrush (Artemisia spp.) for food and shelter during winter, yet few studies have assessed winter habitat selection, particularly at scales applicable to conservation planning. Small changes to availability of winter habitats have caused drastic reductions in some sage-grouse populations. We modeled winter habitat selection by s...