BACKGROUND Ammonium is produced in culture medium due to amino acids degradation and has adverse effect on in vitro culture of embryo. In the current study, the purpose was to evaluate the effects of ammuniom chloride (AC) on in vitro oocyte maturation rate and early embryo development in the sheep and its effect on the expression of Bcl-2. METHODS In vitro maturation (IVM) was performed in t...

In the present study we present evidence that the proportion of complement-receptor lymphocytes (CRL) Is greatly increased in the circulation In most cases of chronic lymphocytic leukemia (CLL). Lymphocytes (> 990/0 pure, 700/0 recovery) were obtained from the peripheral blood of normal Individuals by separation of the mononuclear cells from the leukocyte-enrlched plasma by differential flotati...

Fig. 1. StabilIty of hBNP in whole-blood samples at 25#{176}C (A) and 4#{176}C (B). Samples were collected from three apparently healthy male volunteers (ages25-27 years) Into 50-mL plastic syringesand quickly transferred to ice-chilledplastic tubes containing EDTA-Na, (1.5 g/L) and hBNP-32 (170 ng/L, 49.1 pmol/L).The tubes also contained aprotlnin (500 KIU/mL, ) phosphoramidon 110.9gIL (20 nmo...

OBJECTIVES To compare three methods of labour induction. DESIGN Randomised controlled trial. SETTING Academic hospitals in Johannesburg, South Africa. SUBJECTS Women with intact membranes due for induction of labour. METHOD Randomised, sealed opaque envelopes were used to allocate women to labour induction with extra-amniotic Foley catheter/titrated oral misoprostol solution (N = 174), ...

Studies were made on the physical and chemical properties of polysaccharides synthesized by cell-free extracts of Streptococcus mutans, Streptococcus sanguis, and Streptococcus sp. and their susceptibilities to dextranases. Among the polysaccharides examined, insoluble glucans were rather resistant to available dextranase preparations, and the insoluble, sticky glucan produced by S. mutans OMZ ...