PURPOSE To test patients from southern India for the presence of mutations that most commonly cause Leber congenital amaurosis (LCA) in northern America. METHODS A review of the literature identified 177 unique LCA causing mutations in eight different genes: aryl hydrocarbon receptor interacting protein-like 1 (AIPL1), crumbs homolog 1 (CRB1), cone-rod homeobox (CRX), guanylate cyclase 2D (GU...

PURPOSE Leber congenital amaurosis (LCA) is an early-onset inherited disorder of childhood blindness characterized by visual impairment noted soon after birth. Variants in at least six genes (AIPL1, CRB1, CRX, GUCY2D, RPE65, and RPGRIP1) have been associated with a diagnosis consistent with LCA or early-onset retinitis pigmentosa (RP). Genetically heterogeneous inheritance complicates the analy...

Prostate cancer is a prevalent age-related disease in North America, accounting for about 15% of all diagnosed cancers. We have previously identified lithocholic acid (LCA) as a potential chemotherapeutic compound that selectively kills neuroblastoma cells while sparing normal human neurons. Now, we report that LCA inhibits the proliferation of androgen-dependent (AD) LNCaP prostate cancer cell...

The traditional life cycle assessment (LCA) does not perform quantitative uncertainty analysis. However, without characterizing the associated uncertainty, the reliability of assessment results cannot be understood or ascertained. In this study, the Bayesian method, in combination with the Monte Carlo technique, is used to quantify and update the uncertainty in LCA results. A case study of appl...

We previously demonstrated that ciprofloxacin (CPX), a new quinolone antibiotic, suppresses Cyp3a in the mouse liver by reducing the hepatic level of lithocholic acid (LCA) produced by intestinal flora. The present study investigated the possibility that other antibiotics with antibacterial activity against LCA-producing bacteria also cause a decrease in the LCA level in the liver, leading to r...

An instrument permitting visual testing in white light following the correction of spherical aberration (SA) and longitudinal chromatic aberration (LCA) was used to explore the visual effect of the combined correction of SA and LCA in future new intraocular lenses (IOLs). The LCA of the eye was corrected using a diffractive element and SA was controlled by an adaptive optics instrument. A visua...

Aging is one of the major risk factors of cancer. The onset of cancer can be postponed by pharmacological and dietary anti-aging interventions. We recently found in yeast cellular models of aging that lithocholic acid (LCA) extends longevity. Here we show that, at concentrations that are not cytotoxic to primary cultures of human neurons, LCA kills the neuroblastoma (NB) cell lines BE(2)-m17, S...

Gastrointestinal epithelial barrier loss due to tight junction (TJ) dysfunction and bile acid-induced diarrhea are common in patients with inflammatory diseases. Although excess colonic bile acids are known to alter mucosal permeability, few studies have compared the effects of specific bile acids on TJ function. We report that the primary bile acid, chenodeoxycholic acid (CDCA), and its 7α-deh...

Life Cycle Thinking (LCT) and Life Cycle Assessment (LCA) are the scientific approaches behind modern environmental policies and business decision support related to Sustainable Consumption and Production. For the efficient and reliable application of LCA, it is necessary that LCA studies are based on consistent, transparent, and qualityassured Life Cycle Inventory (LCI) data. To manage and pro...