We evaluated the effectiveness of using Flinders Technology Associates (FTA) filter paper for the polymerase chain reaction (PCR) genotyping of transgenic mice. Tail prick blood sample dried on an FTA filter disc was processed for genomic PCR. It is easy and rapid to prepare DNA templates because the protocol is extraction-free and only requires minimal handling of wash briefly bloodstained FTA...

BACKGROUND Traumatic brain injury (TBI) constitutes the leading cause of posttraumatic mortality. Practically, the major interventions required to treat TBI predicate expedited transfer to CT after excluding other immediately life-threatening conditions. At our center, trauma responses variably consist of either full trauma activation (FTA) including an attending trauma surgeon or a non-trauma ...

As manufacturing tariffs have fallen worldwide, the focus of free trade agreements has shifted towards other issues, including: rules governing foreign investment, e-commerce regulations, trade in services, harmonization of technical standards, sanitary and phyto-sanitary regulations, and the streamlining of customs procedures. Japan and Singapore are undertaking negotiations over this kind of ...

BACKGROUND Syphilis is still a public health problem in Latin America and the Caribbean. Therefore maternal screening is of great importance for public health. AIM To estimate the prevalence of maternal syphilis and to evaluate a rapid diagnostic test for the disease among Bolivian women. MATERIAL AND METHODS A total of 489 women delivering in a Public Hospital in Cochabamba, Bolivia, answe...

Leishmaniasis remains one of the world's most neglected diseases, and early detection of the infectious agent, especially in developing countries, will require a simple and rapid test. In this study, we established a quick, one-step, single-tube, highly sensitive loop-mediated isothermal amplification (LAMP) assay for rapid detection of Leishmania DNA from tissue materials spotted on an FTA car...

Fault tree analysis (FTA) is a widely used method for analyzing a system’s failure logic and calculating overall reliability. However, application of conventional FTA has some shortcomings, e.g. in handling the uncertainties, allowing the use of linguistic variables, and integrating human error in failure logic model. Hence, Fuzzy set theory has been proposed to overcome the limitation of conve...

The ability to archive biological samples for subsequent nucleic acid analysis is essential for tissue specimens and forensic samples. FTA Card is a chemically treated filter paper designed for the collection and room temperature storage of biological samples for subsequent DNA analysis. Its usefulness for the preservation of biological samples for subsequent RNA analysis was tested. Here, we d...

Outpatient departments lie at a critical interface between primary care and acute services. Increasing pressure to ensure efficient and effective health care delivery has resulted in outpatient departments investigating ways to minimise the number of patients failing to attend their scheduled clinic appointments. Failure to attend (FTA) is an expensive and persistent problem worldwide with rate...