BACKGROUND Multiantibiotic-resistant bacteria pose a threat to patients and place an economic burden on health care systems. Carbapenem-resistant bacilli and extended-spectrum β-lactamase (ESBL) producers drive the need to screen infected and colonized patients for patient management and infection control. METHODS We describe a multiplex microfluidic PCR test for perianal swab samples (Acuita...

OBJECTIVES Our aim was to unravel the inactivation pathway of the class A β-lactamase produced by Bacillus licheniformis BS3 (BS3) by clavulanate. METHODS The interaction between clavulanate and BS3 was studied by X-ray crystallography, pre-steady-state kinetics and mass spectrometry. RESULTS The analysis of the X-ray structure of the complex yielded by the reaction between clavulanate and ...

The infections that causes by Pseudomonas aeruginosa is usually responsible for the nosocomial of United States. Occurs in each year approximality 51,000 cases P. , and persons most risk infection are those exposer into equipment hospital has not well desinfection such as ventilation mechanical catheters.Some strains mutate or production β-Lactamase enzymes resistance penicillins.The acts on di...

Infection by Pseudomonas aeruginosa has spread worldwide, with limited options for treatment. The purpose of this study was to investigate metallo-β-lactamase-producing P. aeruginosa strains and compare their genetic profile using samples collected from patients in intensive care units. Forty P. aeruginosa strains were isolated from two public hospitals in Campo Grande, Mato Grosso do Sul State...

Background: Staphylococcus aureus has the ability to develop many efficient mechanisms neutralize them and it become difficult control virulent strains of S. from causing staphylococcal diseases in animals humans. Mostly Staphylococcal have resistant methicillin, β-lactamase ESBL activity sometime vancomycin also. The present study investigated phenotypic genotypic characteristics all 62 isolat...

The AmpC β-lactamases are clinically important because they confer resistance to the narrow-, expandedand the broad-spectrum cephalosporins, the β-lactam-β-lactamase inhibitor combinations and aztreonam. The clinical microbiology laboratories should be able to detect the bacterial strains which produce AmpC, since these strains may appear to be susceptible to a particular β-lactam antibiotic in...

A clinical Providencia stuartii isolate SM662 was recovered from a patient hospitalized in the intensive care unit at the Military hospital, Tunisia. This isolate was resistant to penicillins, cephalosporins, aminoglycosides and fluoroquinolones. A marked in vitro synergy between ceftazidime or cefotaxime and amoxicillin-clavulanic acid on Mueller-Hinton agar plates suggested the presence of an...

The increasing incidence and prevalence of multi-drug resistance (MDR) among contemporary Gram-negative bacteria represents a significant threat to human health. Since their discovery, β-lactam antibiotics have been a major component of the armamentarium against these serious pathogens. Unfortunately, a wide range of β-lactamase enzymes have emerged that are capable of inactivating these powerf...

The emergence of Klebsiella pneumoniae carbapenemases (KPCs), β-lactamases that inactivate "last-line" antibiotics such as imipenem, represents a major challenge to contemporary antibiotic therapies. The combination of ceftazidime (CAZ) and avibactam (AVI), a potent β-lactamase inhibitor, represents an attempt to overcome this formidable threat and to restore the efficacy of the antibiotic agai...