Agaro-oligosaccharides were hydrolytically obtained from agar using hydrochloric acid, citric acid, and cationic exchange resin (solid acid). The FT-IR and NMR data showed that the hydrolysate has the structure of agaro-oligomers. Orthogonal matrix method was applied to optimize the preparation conditions based on -naphthylamine end-labeled HPLC analysis method. The optimal way for oligosacchar...

A rapid and sensitive flow-injection spectrophotometric method is proposed for the determination of sulfadiazine and sulfamethoxazole. This method is based on the diazotization of sulfonamide with sodium nitrite, and a coupling reaction of the diazo-compound with alpha-naphthylamine. The optimum experimental conditions are obtained by using the controlled and weighted centroid simplex method. T...

Six rhizobium (Rhizobium leguminosarum bv. Trifolii TA1, Sinorhizobium meliloti 1021, Mesorhizobium huakuii IFO 15243(T), Ochrobactrum lupini LUP 21(T), Bradyrhizobium japonicum USDA110 and B. elkanii USDA 76) and two Escherichia coli strains (E. coli ATCC 25922 and E. coli HB 101) were compared in respect to polymyxin B and EDTA resistance, as well as bacterial outer membrane (OM) permeability...

An immobilized amino acid napbthylamide substrate for proteinases with tryptic-like specificity was prepared by reacting L-arginine P-naphthylamide with an N-hydroxysuccinimide-activated derivative of agarose. Hydrolysis of the immobilized substrate (AlO-Arg-PNA) was followed by monitoring the increase in fluorescence accompanying the release of /3-naphthylamine. Assays using AIO-Arg-PNA were d...

the side effects associated with the usage of synthetic antidiabetic drugs make it imperative to search for alternative drugs from medicinal plants. therefore, this study was aimed at evaluating the α-amylase and α-glucosidase inhibitory potential of calotropis procera leaf, as well as its possible mode of inhibiting these enzymes. acetone, aqueous and ethanolic extracts of c. procera leaf was ...