Starting from its translation initiation site, the Streptomyces lividans xylanase A signal peptide consists of 41 amino acids. This signal peptide was deleted and successively replaced with one of six signal peptides from other enzymes secreted by S. lividans and by a signal peptide from the outer membrane protein (LamB) of Escherichia coli. Deletion of the xylanase A signal peptide or modifica...

The production of cellulase-free xylanase was investigated with a locally isolated and identified strain, Cellulosimicrobium sp. MTCC 10645 in solid state fermentation. Different fermentation conditions were standardised for the growth and xylanase activity, the optimum being 72 h growth at pH 7.0, cultivation temperature 40°C and substrate to moisture ratio of 1:1.8 (w/v). Among different lign...

A new xylanolytic strain, Paenibacillus favisporus CC02-N2, was isolated from sugarcane plantation fields in Brazil. The strain had a xylan-degrading system with multiple enzymes, one of which, xylanase Xyn30A, was identified and characterized. The enzyme is a single-domain xylanase belonging to family 30 of the glycosyl hydrolases (GH30). Xyn30A shows high activity on glucuronoxylans, with a V...

In this study, forty-two fungi from soil were isolated and tested for their carboxymethyl cellulase (CMCase) and xylanase activities. From all isolates, the fungal isolate FR60, which was identified as Aspergillus aculeatus Iizuka, showed high activities in both CMCase and xylanase with 517 mU/mg protein and 550 mU/mg protein, respectively. The crude enzyme from A. aculeatus Iizuka FR60 could h...

BACKGROUND Aspergillus fumigatus R1 produced xylanase under submerged fermentation which degrades the complex hemicelluloses contained in agricultural substrates. Xylanases have gained considerable attention because of their tremendous applications in industries. The purpose of our study was to purify xylanase and study its biochemical properties. We have predicted the secondary structure of pu...

Abstract Hydrothermal methods are commonly applied in pretreatment of lignocellulose for conversion to biofuels and chemicals. This partially disassembles solubilizes hemicelluloses lignin, releasing sugars phenolic compounds, which potential inhibitors the subsequent enzymatic saccharification step. In this work, inhibitory effect soluble phenolics hydrothermal liquor on canonical Trichoderma ...

Xylanase is a major enzyme used in the paper and pulp industries for biobleaching applications. There are possibilities for xylanase with better properties suitable for industrial applications. This paper focused on a potential xylanolytic fungus, Trichoderma viride VKF-3, obtained from a mangrove soil sample. Optimum conditions for xylanase production were tested by culturing T. viride VKF3 un...

A 2 × 2 factorial experiment was conducted to investigate the effect of dietary protease (0 and 200 mg/kg) and xylanase (0 and 200mg/kg) in reducing manure odor and NH(3) from finisher pigs. Sixteen pigs were assigned to 1 of 4 dietary treatments, (i) basal diet, (ii) basal diet + xylanase, (iii) basal diet + protease, or (iv) basal diet + xylanase + protease, for 24 d. The manure samples from ...

Solid-state fermentation of sugarcane bagasse by Penicillium citrinum MTCC 2553 was optimized to maximize the yield of xylanase. Preliminary experiments carried out with various lignocellulosic materials revealed sugarcane bagasse to be the most suitable substrate for producing xylanase. Response surface methodology was used in the optimization. Xylanase activity was maximized in a 5-day batch ...