The aim of this study is to investigate the effects of taurine treatment on cell viability and proliferations in BHK-21 cells. In this study, BHK-21 cells were seeded at a density of 50.000/ml per well in 96 well plates in medium composed of Glasgow-Minimum Essential Medium (GMEM). The cells were either left untreated (control) or treated with different concentrations of taurine for 24 h. XTT c...

Rule-based programming paradigm is omnipresent in number of engineering domains. However, there are some fundamental semantical differences between it, and classic programming approaches. No generic solution for using rules to model business logic in classic software has been provided so far. In this paper a new approach for Generalized Rule-based Programming (GREP) is given. It is based on a u...

This paper presents an extension of classical attributive logic using atomic values of attributes. In the proposed logic set values are allowed and various relational symbols are used to form atomic formulae. The proposed language provides a concise and elegant tool for design, implementation and verification of rule-based systems.

Chitosan/silk fibroin (CS/SF) blend films were prepared and evaluated for feasibility of using the films as biomaterial for skin tissue engineering application. Fourier transform infrared spectroscopy and differential scanning calorimetry analysis indicated chemical interaction between chitosan and fibroin. Chitosan enhanced β-sheet conformation of fibroin and resulted in shifting of thermal de...

Aim: To evaluate the cytotoxicity of several storage media (coconut water, whole milk, isotonic solution, Hank ́s Balanced Salt Solution and tap water) using a multiparametric cytotoxicity analysis employing 3T3 cells. Methods: Plates containing confluent 3T3 fibroblasts were exposed to the various media for 24h, at 37°C with 5% CO2, and cell viability was evaluated by a multiparametric assay as...

Purpose: Previous studies have shown that cannabinoid 2 agonists anticancer effects on different cancer cell lines. However, the effect of L-759,633 C6 and SH-SY5Y lines is still unclear. The current study was designed to investigate potential cytotoxic a agonist Material Methods: In this study, were used. For each line two groups prepared examine glioma death. It not applied any treatment cell...

Candidiasis is an opportunistic infection affecting immunosuppressed and hospitalized patients, with mortality rates approaching 40% in Colombia. The growing pharmacological resistance of Candida species the emergence multidrug-resistant auris are major public health problems. Therefore, different antimicrobial peptides (AMPs) being investigated as therapeutic alternatives to control candidiasi...

Five known compounds, four triterpenoids [(lup-20(30)-ene-3 ,29-diol (1), lup-20(29)-ene-30-hydroxy-3one-(2), – taraxastanonol 3, -sitosterol 4] and 4’ –Omethylepigallocatechin 5 were isolated from Elaeodendron transvaalense bark ethanol extract. This plant is traditionally used in Southern Africa for the treatment of various ailments including sexually associated diseases. Cytotoxicity of the ...

This study quantifies the production of single and mixed biofilms of Candida albicans and Staphylococcus aureus to determine if such mixed biofilms have synergistic effects. Assays were performed using polystyrene microtitre plates of 96 wells, metabolic activity was measured by the enzymatic reduction of a tetrazolium salt (XTT) and colorimetric changes were measured at 490 nm. Confocal scanni...

We explore a series of Zn and N codoped TiO2 thin films grown using chemical vapor deposition. Films were prepared with various concentrations (0.4-2.9 at. % vs Ti), their impact on superoxide formation, photocatalytic activity, bactericidal properties determined. Superoxide (O2•-) formation was assessed 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenylamino)carbonyl]-2H-tetrazolium sodium sa...