Ethanol has been used, or proposed for use, as a process fluid or separation agent for a number of biorefining operations. These include oil extraction, solvent exchange, displacement drying, protein extraction, blood plasma fractionation, shellac deposition, protein film deposition, corn zein protein extraction, corn total protein extraction and wheat gluten protein from starch physical separa...

Barley is an important crop for the production of malt and beer. However, crops such as rice and wheat are rarely used for malting. α-amylase is the key enzyme that degrades starch during malting. In this study, we compared the genomic properties, gene copies, and conserved promoter motifs of α-amylase genes in barley, rice, and wheat. In all three crops, α-amylase consists of four subfamilies ...

Starch is a main component of wheat flour, which, besides being an occupational allergen can also induce irritative symptoms in the airways. A purified starch product (cornstarch glove powder) was used to investigate whether starch alone could induce airway inflammation. The aim of the study was to investigate a role for starch in wheat flour-induced airway inflammation. Ten healthy individuals...

It is well known from light microscope studies of potato starch that the granules formed in a constant environment (of light and temperature) have a ring formation indistinguishable from that of granules formed under field conditions. Electron microscope studies have confirmed that normal potato starch granules have a fine shell structure not usually resolved by the light microscope, and also t...

The manufacturing of ethanol from cereal crop by enzymatic conversion of starch to fermentable glucose and fermentation by yeast is now a well established process. The first phase of the process is the addition of alpha-amylases to the liquefaction of the milled grains. Alpha-amylases hydrolyses the α-1,4-glucosidic bonds to reduce the viscosity of gelatinized starch producing soluble starch an...

To examine the role of isoamylase1 (ISA1) in amylopectin biosynthesis in plants, a genomic DNA fragment from Aegilops tauschii was introduced into the ISA1-deficient rice (Oryza sativa) sugary-1 mutant line EM914, in which endosperm starch is completely replaced by phytoglycogen. A. tauschii is the D genome donor of wheat (Triticum aestivum), and the introduced fragment effectively included the...

Polyhydroxybutyrate producing bacteria from garden soil were isolated and characterized for their morphological, biochemical properties. Based on their 16S rRNA gene sequences, they were identified as Bacillus mycoides DFC1, Bacillus cereus DC1, Bacillus cereus DC2, Bacillus cereus DC3 and Bacillus cereus DC4. The bacteria were screened for PHB production and compared for the intensity of fluor...

The controlling importance of microstructure in deep-fat frying has been greatly recognized when studying oil absorption mechanisms. This knowledge should be considered when designing novel fabricated products and the use of non-destructive microscopic techniques could be helpful in doing so. Food products formulation by the combination of components derived from raw agricultural materials, or ...

Wheat starch degradation requires the synergistic action of different amylolytic enzymes. Our spatio-temporal study of wheat α-amylases throughout grain development shows that AMY3 is the most abundant isoform compared with the other known α-amylases. Endosperm-specific over-expression of AMY3 resulted in an increase of total α-amylase activity in harvested grains. Unexpectedly, increased activ...

Gelatinized wheat, potato and waxy maize starches were treated enzymatically in order to increase the degree of branching of the amylopectin fraction and thereby change the starch degradation profile towards a higher proportion of slowly digestible starch (SDS). The materials were characterized by single-pulse (1)H HR-MAS NMR spectroscopy and in vitro digestion profile according to the Englyst ...