This work reports the development and application of a negative ion mode online LC-ESI-MS method for studying the effect of water deficit on the carbohydrate content of Lupinus albus stems, using a porous graphitic carbon (PGC) stationary phase and an ion trap mass spectrometer. Using this method, separation and detection of several water soluble carbohydrates, ranging from mono-, di-, and olig...

Opuntia dillenii (Ker Gawl.) Haw., a well-known member of the Cactaceae family, played an important role in agriculture and medicine. In this study, extraction conditions physico-chemical properties water-soluble polysaccharides (OMP) from mucilage were investigated. The cactus cladodes used study collected Binh Thuan province, Vietnam. optimum water with conventional heating included at temper...

Recent developments in supercritical fluid chromatography (SFC) have highlighted the applicability of this technique for analysis highly polar compounds. The combination stationary phases and CO2-based mobile with an increasing presence liquid co-solvent (up to 100%) has enabled further expand application field SFC towards a variety samples such as endogenous metabolites, plant extracts, water-...

Effects of soaking and cooking methods on physicochemical characteristics, nutrients and antinutrients in twenty soybean genotypes were studied. Batches of seeds were soaked for 18 h in distilled water, 1% citric acid and 2% sodium bicarbonate solutions at room temperature and then boiled in water. Raw soybean genotypes exhibited 36.5-43.2% protein, 20.7-22.2% oil, 2.58.3% total soluble sugars,...

The nutritional composition of different acorn species (Q. pyrenaica, Q. robur, and ilex) harvested in Peneda-Gerês Park, Braga (north Portugal) was determined after being dehulled by methods (manually, thermally scalding with boiling water, drying at 30 °C). Ash protein contents remained unaltered, but the fat carbohydrate increased and/or decreased depending on dehulling method Quercus spp. t...

Changes in cold hardiness, carbohydrate content and β-amylase gene expression were monitored in the shoots of the highbush blueberry (Vaccinium corymbosum L.) cultivars 'Sharpblue' and 'Jersey' during cold acclimation (CA) and deacclimation (DA). The seasonal patterns were similar in both cultivars, but the levels of cold hardiness determined by electrolyte leakage analysis were significantly d...

Fenugreek seeds (raw, soaked and germinated) were analyzed for their chemical composition. Raw fenugreek seeds contained higher amount of dietary jiber 46.50% followed by 42.12% in soaked seeds and 32.50% in germinated seeds. Soaking reduced the level of total soluble sugars, reducing sugars, nonreducing sugars, dietary fiber and improved the protein and starch digestibility and availability of...