نتایج جستجو برای: vigs
تعداد نتایج: 345 فیلتر نتایج به سال:
Alternaria alternata (tobacco pathotype) is a necrotrophic fungus causing severe losses in Nicotiana species by infection of mature leaves. Similar to what has been observed in cultivated tobacco, N. tabacum, young leaves of wild tobacco, N. attenuata, were more resistant to A. alternata than mature leaves, and this was correlated with stronger blue fluorescence induced after infection. However...
Calcium-dependent protein kinases (CPKs) are important Ca2+ signalling components involved in complex immune and stress signalling networks; but the knowledge of CPK gene functions in the hexaploid wheat is limited. Previously, TaCPK2 was shown to be inducible by powdery mildew (Blumeria graminis tritici, Bgt) infection in wheat. Here, its functions in disease resistance are characterized furth...
Virus-induced gene silencing (VIGS) was used as a tool for functional analysis of cell wall-associated genes that have been suggested to be involved in leaf abscission. Tobacco rattle virus is an effective vector for VIGS in tomato (Lycopersicon esculentum). Silencing was more efficient when the plants were grown at 22 degrees C than when they were grown at 20 degrees C or 25 degrees C. The pho...
The Ethylene-Responsive Factors (ERFs) comprise a large family of transcriptional factors that play critical roles in plant immunity. Gray mold disease caused by Botrytis cinerea, a typical necrotrophic fungal pathogen, is the serious disease that threatens tomato production worldwide. However, littler is known about the molecular mechanism regulating the immunity to B. cinerea in tomato. In th...
Lycopene biosynthesis in tomato (Solanum lycopersicum) fruits has been proposed to proceed through a poly-cis pathway catalyzed by phytoene synthase (PSY), two desaturases (phytoene desaturase [PDS] and ζ-carotene desaturase [ZDS]), and two cis-trans isomerases (ζ-carotene isomerase [ZISO] and prolycopene isomerase [CrtISO]). The mechanism of action of these enzymes has been studied in Escheric...
Bri1-associated kinase 1 (BAK1)-interacting receptor-like kinase (BIR) proteins have been shown to play important roles in regulating growth and development, pathogen associated molecular pattern (PAMP)-triggered immunity (PTI) responses, and cell death in the model plant, Arabidopsis thaliana. We identified four BIR family members in tomato (Solanum lycopersicum), including SlBIR3, an ortholog...
Modern Concepts & Developments in Agronomy Construction of VIGS Vector Root-knot Resistant WRKY64 Gene Rapeseed Addition Line EE Ping Yu1, Lihua Zhao1, Chao Dong1, Xiaofang Zhang3, Hongmei Luo1, Holger Budahn2, and Shaosong Zhang1* 1Biotechnology Genetic Germplasm Institute, Yunnan Academy Agricultural Sciences, Kunming 650223, Yunnan, China 2Julius Kühn-Institut Institute for Breeding Research...
هدف: در این پژوهش، بهمنظور بررسی تاثیر خاموشی بر بیان ژن کلیدی DBOX (که آنزیم نهایی بیوسنتز دو آلکالوئید سنگوینارین و پاپاورین را کد می‌کند)، از فن VIGS در گونه ای از خشخاش (Papaver somniferum L.) استفاده شد. مواد و روش‌ها: قطعه 350 جفت بازی از توالی ژن DBOX (در محدوده bp1462-1112) براساس تولید بیشترین تعداد siRNA با طول 21 نوکلئوتید انتخاب شد....
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