Cryptococcus neoformans MATalpha and MATa pheromones were amplified by direct PCR. Nucleotide sequence analyses revealed unique restriction enzyme sites. Sixty strains were used to devise a restriction fragment length polymorphism typing scheme that yielded three variety-specific patterns. Additionally, pheromone-specific PCR allowed easier identification of diploid C. neoformans strains than f...

Twenty-eight enteroviral isolates obtained from various clinical specimens were typed by Lim-Benyesh-Melnick (LBM) pool-based neutralization, PCR-restriction fragment length polymorphism (RFLP), and partial sequencing of the VP1 region of the enteroviral genome. Sequencing was found to be a good alternative to LBM typing, while PCR-RFLP was inadequate for identification of enteroviral isolates.

background: vitiligo is an acquired skin disorder that selectively destroys melanocytes in epidermis with an unknown etiology.   objective: to investigate the exon 1 a49g polymorphism of cytotoxic t lymphocyte antigen-4 (ctla-4) gene in vitiligo patients.   methods: the a49g polymorphism was detected by polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) method in 101 ...

During 2013-2015, prevalence of cutaneous leishmaniasis in war-affected Waziristan areas was 3.61% by PCR. Youths (1-15 years of age) were more susceptible. Internal transcribed spacer 1 PCR followed by restriction fragment length polymorphism analysis identified Leishmania tropica in 215 samples and Leishmania major in 6 samples.

OBJECTIVES The aim of the present work was to standardise a PCR-Restriction Fragment Length Polymorphism analysis (PRA) as a tool to detect the mycobacteriologic composition of lepromas from leprosy patients used in the production of lepromin to improve the quality of the Mitsuda test. DESIGN PCR-Restriction Fragment Length Polymorphism analysis using hsp65 and rpoB genes were applied to 11 r...

objective: to determine the possible association between the m235t variant of angiotensinogen gene and preeclampsia in iranian preeclamtic women with hypertension during pregnancy. mand methods: during a case control study, we used polymerase chain reaction-based restriction fragment length polymorphism (pcr-rflp) analysis to investigate the association between m235t polymorphism in preeclamtic...

OBJECTIVE To clarify whether analysis of loss of heterozygosity (LOH) on chromosome 16q is possible using DNA isolated from fine needle aspiration specimens, a simulation study was performed using resected biopsy or mastectomy specimens of 37 breast carcinomas and 3 fibroadenomas. STUDY DESIGN A highly polymorphic (AC)n repeat region on the D16S305 locus on chromosome 16q24 was amplified in t...

Background: Macrophage migration inhibitory factor (MIF) is a key pro-inflammatory cytokine that is secreted by active macrophages accumulated in ectopic tissue of endometriosis. It involves in pathophysiological events of endometriosis such as angiogenesis, cell proliferation and it can stimulate the synthesis of PGE2 that are necessary for survival and establishment of ectopic endometriosis t...