Alkaline transfer to nylon membranes is a widely used modification of the Southern blot procedure (1). However, several reports describe decreased hybridization efficiency with alkaline transfer (2,3,4). Shorter exposure time to alkali, or lower alkali concentration have been suggested to increase hybridization efficiency. We report an example of altered DNA banding following alkali transfer co...

Soil fungi play a fundamental role in the cycling of nutrients and tree nutrition forests. As mycorrhizal are closely associated with their host trees provide them essential base cations phosphorus, we hypothesize that they actively mine soil minerals transfer nutritive content to host. Therefore, expected be important colonizers mineral surfaces relative other fungal trophic guilds. To test th...

this study was conducted to determine the chemical composition and degradability characteristics of a pasture plant salsola tomentosa by nylon bags method .the sampling was performed from the northern region of plain's gonabad city in three growth stages (vegetative, flowering and seeding). the samples dried in air, were milled and their chemical composition were determined. to determine the de...

A novel and facile approach to prepare hybrid nanoparticle embedded polymer nanofibers using pressurised gyration is presented. Silver nanoparticles and nylon polymer were used in this work. The polymer solution's physical properties, rotating speed and the working pressure had a significant influence on the fibre diameter and the morphology. Fibres in the range of 60-500nm were spun using 10wt...

Feeding cereal grain to cattle is common practice for optimal beef and milk production. High concentrations of starch other soluble carbohydrates may cause acidosis. Information on the effect processing protein degradability in rumen are scarce. This study was determine ruminal degradation patterns grains steam pelleting crude (CP) rumen. The pattern dry matter (DM), starch, CP ground pelleted ...