Cryptosporidium parvum (C. parvum) infection is one of the main causes diarrhea in calves. The current study assessed role blood biomarkers (acute-phase proteins (APPs), procalcitonin, neopterin, cytokines, and oxidative stress pathogenesis, diagnosis, prognosis calves naturally infected with C. parvum. Fifty-seven calves, aged from 10 to 45 days, were detected positive for allotted into diseas...

A differential agar medium for the identification of Ureaplasma urealyticum in primary cultures of clinical specimens is described. The differential medium (no. A7) is specific for the identification of U. urealyticum and other members of the genus Ureaplasma. Large-colony, classical Mycoplasma and Acholeplasma species and Proteus L colonies are unreactive on this differential medium. The mediu...

Adherence of Ureaplasma urealyticum cells to eukaryotic cell monolayers was quantified using the Bertholet assay to monitor ammonia produced from urea by ureaplasma urease. Adherence was abolished by pre-treatment of ureaplasmas with HeLa cell extracts and inhibited to varying degrees by pretreatment of the ureaplasmas with N-acetylneuraminic acid, specific antisera and monoclonal antibodies. T...

mycoplasma and ureaplasma species are the most serious contaminants of cell cultures and this remains one of the major problem encountered in biological research using cell cultures. the aim of this study was to investigate the rate of mycoplasma and a total of 100 different cell culture specimens including, r228, hela, vero, mdck, l20, rd and hep2 were collected. then the specimens were invest...

Chorioamnionitis is a major risk factor for preterm birth and an independent postnatal morbidity which currently successful therapies are lacking. Emerging evidence indicates that the timing duration of intra-amniotic infections crucial determinants stage developmental injury at birth. Insight into dynamical changes organ after onset chorioamnionitis revealed novel therapeutic windows opportuni...