This paper presents an enzyme-based method for the determination of urea in aqueous samples using crude urease from Citrullus lanatus seeds. The is based on enzymatic hydrolysis Urea into ammonia (NH4) and carbon dioxide (CO2) by urease. Ammonia formation determined spectrophotometrically after nesslerization then quantitatively related to concentration via a calibration curve. Reaction conditi...

Urea is an added value chemical with wide applications in the industry and agriculture. The release of urea waste to environment affects ecosystem health despite its low toxicity. Online monitoring for industrial environmental unaddressed challenge. Electroanalytical techniques can be a smart integrated solution online if sensors overcome major barrier associated long-term stability. Mixed meta...