It is difficult to genetically manipulate the medically and biotechnologically important genus Clostridium due to the existence of the restriction and modification (RM) systems. We identified and engineered the RM system of a model clostridial species, C. acetobutylicum, with the aim to allow the host to accept the unmethylated DNA efficiently. A gene CAC1502 putatively encoding the type II res...

Introduction: The coronavirus disease 2019 (COVID-19) pandemic caused by Severe Acute Respiratory Syndrome-Coronavirus 2 (SARS-CoV-2) had spread worldwide. Nurses as health care workers (HCWs) are at great risk of contracting the virus, so they prioritized for accepting Covid-19 vaccination. This study aimed to determine intention nurses accept covid-19 Method: online survey was given all in Ea...

This thesis evaluates techniques for improving the performance of three architecturally different web servers. We study strategies for effectively accepting incoming connections under conditions of high load. The experimental evaluation shows that the method used to accept new connection requests can significantly impact server performance. By modifying each server’s accept strategy, we improve...

We are most grateful for the comments made by Earl (2014) on our paper, Frey and Gallus (2014). In particular, we are pleased that we can fully agree on the fundamental point we wanted to make: decisions by individuals (as well as by organizational units) do not map directly and in an unchanged way to the macro level. Rather, the aggregation process linking the microand the macro-level must be ...

In 1937, Ronald Coase argued that firms will exist only in environments in which firms perform better than markets could. To create space for firms, Coase suggested that some environments might be plagued by “transaction costs” that cause markets to perform poorly. Coase’s paper was to become the cornerstone of the economic theory of the firm (i.e., the “make or buy” decision: which activities ...

This paper proposes and evaluates a model of supply chains as networks of auctions. In this model, companies are represented according to the first level of the Supply Chain Council’s SCOR model and the trading strategy of the agents is adapted from a model proposed by Steiglitz and colleagues. Specifically, the highest level of SCOR treats a company as comprising three functions, namely Source...