Regulated expression of the Escherichia coli dam gene has been achieved with the araBAD promoter lacking a ribosome binding site. Cultures of dam mutants containing plasmid pMQ430 show no detectable methylation in the absence of arabinose and complete methylation in its presence. Dam methyltransferase is a substrate for the Lon protease.

Invasion of intestinal epithelial cells is a critical step in Salmonella infection and requires the expression of genes located in Salmonella pathogenicity island 1 (SPI-1). A key factor for SPI-1 expression is DNA adenine (Dam) methylation, which activates synthesis of the SPI-1 transcriptional activator HilD. Dam-dependent regulation of hilD is postranscriptional (and therefore indirect), ind...

Due to the high attenuation of radio frequency (RF) signals in underground mines and the statutory power restriction of communication gadgets especially for coal mines, the wireless communication systems have restricted applications in the underground mines. The paper discusses different radio frequency communication techniques being employed for Indian underground mines. Experiments were condu...

Salmonella strains that lack or overproduce DNA adenine methylase (Dam) elicit a protective immune response to different Salmonella species. To generate vaccines against other bacterial pathogens, the dam genes of Yersinia pseudotuberculosis and Vibrio cholerae were disrupted but found to be essential for viability. Overproduction of Dam significantly attenuated the virulence of these two patho...

Due to its unique sensitivity to hydrogen protons, magnetic resonance sounding (MRS) is the only geophysical method that directly detects water and can provide nondestructive information on subsurface aquifer properties. The relationship between the surface MRS signal and the location and characteristics of aquifers using large-coil (typically 50-150 m) sensors has been discussed based on forwa...

Many coal mine companies have established underground safety monitor and control systems. However, the safety management information can only be displayed on the 2D maps or as reports. In this paper, a new method that combines the VR technologies with underground mine safety monitoring technologies was explored. A desktop virtual reality program for underground coal mine, called VRLane, was dev...

This study examined the effect of underground water on reproductionand growth-related hormones in blue gourami males under non-reproductive and reproductive conditions. An increase in the percentage of males building nests under the highest percentage of underground water were compared to fish that maintained a lower percentage of underground water in the first two days. The % Gonado-somatic in...

Wireless underground sensor networks (WUSNs) constitute one of the promising application areas of the recently developed wireless sensor networking techniques. The main difference between WUSNs and the terrestrial wireless sensor networks is the communication medium. The propagation characteristics of electromagnetic (EM) waves in soil and the significant differences between propagation in air ...

Studies conducted on surface coal mining equipment have identified whole-body vibration as a significant hazard. Operators of underground mobile equipment, particularly shuttle cars and transport vehicles, are likely to be exposed to significant levels of whole-body vibration. To date, measuring whole-body vibration from underground mining mobile equipment has been difficult due to the strict g...