Pseudolaric acid B (PLAB) is one of the major bioactive components of Pseudolarix kaempferi. It has been reported to exhibit inhibitory effect on cell proliferation in several types of cancer cells. However, there is no report elucidating its effect on glioma cells and organ toxicity in vivo. In the present study, we found that PLAB inhibited growth of U87 glioblastoma cells in a dose-dependent...

Stemness genes, including NANOG, which have been reported to play a significant role in embryonic stem cells (ESCs), are purported to be expressed in specific human tumor types. In the present study, we explored the expression of NANOG in gliomas to demonstrate its key roles in maintaining the undifferentiated state of glioma cells. Brain tumor stem cells (BTSCs) were isolated from the human gl...

Despite therapeutic advances, the prognosis of patients diagnosed with malignant glioma has not improved in recent years. In particular, the molecular mechanisms that mediate glioma invasion remain poorly understood. The importance of ID1 in promoting tumor invasion and metastasis has recently emerged and a role for ID1 as a possible molecular marker of tumor aggressiveness has been proposed. T...

We investigated the effect of pannexin-1 (PANX-1) on the release of glutamate and cytokines in U87 human malignant glioma (U87-MG) cells using small interfering RNA (siRNA) transfection and adenosine triphosphate/lipopolysaccharide treatment. PANX-1 is a new family member of the gap junction proteins, and is permeable to ions, metabolic molecules, second messengers, and neurotransmitters, among...

Glioblastomas exploit various molecular pathways to promote glutamate- dependent growth by activating the AMPA (2-amino-3-(3-hydroxy-5-methyl-isoxazol-4-yl) propanoic acid) receptor, the group II metabotropic glutamate receptor, mGluR, and the epidermal growth factor receptor, EGFR. We hypothesized that targeting more than one of these pathways would be more effective in inhibiting glutamate-de...

This study aimed to investigate the anticarcinogenic and genotoxic damage potentials of malic acid on human fibroblast cells (HDFa) glioblastoma (U87-MG) cell lines. MTT viability LDH release assays were performed understand cytotoxic features different Also, Hoechst 33258 fluorescent staining was used monitor nuclear abnormalities including micronucleus, lobbed, notched structures. Furthermore...

Amaç: Bu çalışmada, glioblastoma hücre hattında (U87) siklopamin ve temozolomid (TMZ) tedavisinin ayrı kombine olarak uygulanmasından sonra miR-20a ekspresyonunun araştırılması bu miRNA’nın tedavisindeki etkinliğinin değerlendirilmesi amaçlanmıştır. Gereç Yöntem: U87 MG, tedaviden önce 35 mm'lik kültür kabı üzerine yerleştirildi çoğaltıldı. Çalışma kapsamında 7 grup oluşturuldu: Grup 1: Kontrol...

Glioblastoma (GBM), the most common and aggressive brain tumor in the central nervous system, remains a lethal diagnosis with a median survival of < 15 months. Aberrant expression of the TRPM7 channel has been linked to GBM functions. In this study, using the human GBM cell line U87, we evaluated the TRPM7 activator naltriben on GBM viability, migration, and invasiveness. First, using the whole...

Glioblastoma, the most malignant form of brain cancer, is responsible for 23% of primary brain tumors and has extremely poor outcome. Confounding the clinical management of glioblastomas is the extreme local invasiveness of these cancer cells. The mechanisms that govern invasion are poorly understood. In order to gain insight into glioblastoma invasion, we conducted experiments on the patterns ...

Plant polyphenols have been highlighted not only as chemopreventive, but also as potential anticancer substances. Flavones are a subclass of natural flavonoids reported to have an antioxidant and anticancer activity. The aim of our study was to evaluate antioxidant and anticancer activity of seventeen trihydroxyflavone derivatives, including apigenin (API) and baicalein (BCL). Also, we wanted t...