This study aimed to investigate the potential use of microalgae Chlorella sorokiniana and Scenedesmus quadricauda for bioremediation olive mill wastewater (OMW), which is a major environmental issue waste product oil production. The investigated effects different dilutions (10% 50%) OMW on growth their ability remove phenolic component hydroxytyrosol (OH-Tyr) enhance antioxidant properties. res...

Oculocutaneous albinism (OCA) is an inherited disorder affecting the visual system and skin pigmentation. Our aim was to evaluate genetic clinical heterogeneity in a cohort of Slovenian paediatric patients with clinically suspected OCA using advanced molecular-genetics approach. In as much 20 out 25 patients, variants explaining their phenotype were identified. The great majority (15/25) had TY...

Studies on the single Cu protein galactose oxidase (68kDa; 639 amino acids) from Fusarium NRRL 2903 are described. The Cu is coordinated in a square based pyramid by Tyr-272, Tyr-495 (axial), His-496, His-581 and H20 (the substrate binding site), and the enzyme functions as a 2-equivalent oxidase, 0, + H,O,, with oxidation of primary alcohol substrates RCH20H to RCHO. The active enzyme has a co...

Most bacteria and eukarya contain an enzyme capable of specifically hydrolyzing D-aminoacyl-tRNA. Here, the archaea Sulfolobus solfataricus is shown to also contain an enzyme activity capable of recycling misaminoacylated D-Tyr-tRNATyr. N-terminal sequencing of this enzyme identifies open reading frame SS02234 (dtd2), the product of which does not present any sequence homology with the known D-...

PURPOSE Oculocutaneous albinism (OCA) is a group of autosomal recessive disorders characterized by deficient synthesis of melanin pigment and associated with common developmental abnormalities of the eye. It is one of the major causes of childhood blindness in India. The disease is common among an ethnic group (Tili) of Eastern India, which represents about 12.56% of the Bankura district popula...

Cyclooxygenases (COXs) are heme-containing sequence homodimers that utilize tyrosyl radical-based catalysis to oxygenate substrates. Tyrosyl radicals are formed from a single turnover of substrate in the peroxidase active site generating an oxy-ferryl porphyrin cation radical intermediate that subsequently gives rise to a Tyr-385 radical in the cyclooxygenase active site and a Tyr-504 radical n...

Injury of articular cartilage leads to an imbalance in tissue homeostasis, and due the poor self-healing capacity affected often exhibits osteoarthritic changes. In recent years, injectable highly tunable composite hydrogels for engineering drug delivery have been introduced as a desirable alternative invasive treatments. this study, we aimed formulate by combining different concentrations hyal...

To study its contribution to the assembly of the green plant manganese stabilizing protein (MSP) into photosystem II (PSII), tyrosine residues were specifically acetylated using N-acetylimidazole (NAI). In soluble MSP, three groups of Tyr residues could be differentiated by NAI acetylation: approximately 5 (actually approximately 5.2) Tyr residues could be easily acetylated (superficial), 1-2 T...

When T cells are activated via the T cell receptor (TCR) complex a number of cellular substrates, including some cell surface proteins, become phosphorylated on tyrosine (Tyr) residues. Phosphorylation of cytoplasmic Tyr renders these cell surface receptors competent to interact with proteins that link cell surface receptors to protein in the intracellular signaling pathways. Here we show that ...

BACKGROUND/AIMS The biological effects of periodic mechanical stress on chondrocytes have been studied extensively over the past few years. However, the mechanisms underlying chondrocyte mechanosensing and signaling in response to periodic mechanical stress remain to be determined. In the current study, we examined the effects of focal adhesion kinase (FAK) signaling on periodic mechanical stre...