Purpose: To evaluate the role of miR-20a in sevoflurane (SEV)-induced cognitive impairment rats and to elucidate mechanism action.
 Methods: A SEV-induced rat model was developed. The Morris water maze test fear assay were carried out assess impaired learning memory. cellular SEV-impaired developed level measured animal models. TUNEL staining immunoblot performed determine SEV effec...

Abstract There is a relationship between person’s diet and the development prevention of some cancers. Carotenoids are found as various natural pigments in many fruits vegetables. Studies on carotenoids their potential roles carcinogenesis increasing importance day by day. In this study, we aimed to determine cytotoxic genotoxic effects capsanthin, carotenoid compound, human prostate cancer cel...

Several studies have reported the occurrence and significance of programmed cell death (apoptosis) of testicular germ cells in mammals. In those studies, apoptotic germ cells were identified by morphological criteria or by in situ end labeling (TUNEL) and were enumerated from histological sections by semi-quantitative and time-consuming techniques. In the present study, we have established a fl...

BACKGROUND Dysplastic nodules (DNs) recently have been identified as preneoplastic lesions of hepatocellular carcinoma (HCC). To test an alternative hypothesis regarding DN development, in which we have suggested that DNs develop as an infiltrating clonal expansion in advance of, or parallel to cirrhosis, the authors investigated the rates of apoptosis and proliferation in human hepatocarcinoge...

We have examined the consequences of Ultraviolet-B (UV-B) irradiation in the thallus of the lichen Physcia semipinnata (J.F.Gmel) in terms of cell viability and apoptotic-like formation. UV-B induced oligonucleosomal DNA fragmentation was detected by TUNEL assay and this is the first study showing DNA fragmentation in thalli. The intensity of TUNEL-positive cells after exposure to UV-B at doses...

The growth plates of the femoral head of Japanese white rabbits aged 5, 10, 15 and 20 weeks were stained for apoptotic and proliferating chondrocytes using the TUNEL and PCNA antibody staining techniques. Both TUNEL- and PCNA-positive chondrocytes were detected in all of the specimens. The positive ratios of both stainings were calculated for the whole plate and for the resting, proliferating a...