Melinjo (Gnetum gnemon L.) seed extract (MSE) and its active ingredient gnetin C (GC), a resveratrol dimer, have been shown to possess a broad spectrum of pharmacological activities. In this study, we investigated the antitumor activity of MSE and GC using human and murine tumor cell culture models in vitro. The antitumor activity of GC was compared with trans-resveratrol (tRV), a stilbenoid po...

OBJECTIVES We aimed to identify risk factors for acute pulmonary events in children and adolescents in the Pulmonary Hypertension and the Hypoxic Response in SCD (PUSH) study. METHODS Patients with hemoglobin SS (n = 376) and other sickle cell genotypes (n = 127) aged 3-20 yrs were studied at four centers in a cross-sectional manner. A subgroup (n = 293) was followed for a median of 21 months...

BackgroundExercise stress echocardiography (SE) is increasingly recognized as a useful tool for assessment of pulmonary hypertension (PH) in patients with sickle cell disease (SCD). However, while the prognostic significance resting systolic artery pressure (SPAP) well established and global longitudinal right ventricular free wall strain (RV FWS) has been identified an independent marker PH, l...

Abstract Background According to current guidelines, the diagnosis of pulmonary hypertension (PH) relies on echocardiographic probability followed by right heart catheterization. How echocardiography predicts PH recently re-defined a mean artery pressure (mPAP) >20 mmHg instead ≥25 and vascular disease defined resistance (PVR) >3 or >2 Wood units has not been established. M...

Abstract Funding Acknowledgements Type of funding sources: Public hospital(s). Main source(s): International Medical Research and Development Fund Background Measuring pulmonary vascular resistance (PVR) is essential for diagnosing hypertension. PVR can be estimated from a simple ratio tricuspid regurgitation velocity (TRV) to time-velocity integral flow through right ventricular outflow tract ...

The development of high-throughput technologies allows for evaluating gene expression at the whole-genome level. Together with proteomic and metabolomic studies, these analyses have resulted in the identification of plant genes whose function or expression is altered as a consequence of pathogen attacks. Members of the Tomato yellow leaf curl virus (TYLCV) complex are among the most important p...

Transferrin (Tf) receptor-variant Chinese hamster ovary cells have been isolated by selection for resistance to two Tf-toxin conjugates. The hybrid toxins contain Tf covalently linked to ricin A chain or a genetically engineered diphtheria toxin fragment. The Tf-receptor-variant (TRV) cells do not have detectable cell-surface Tf receptor; they do not bind fluorescein-Tf or ~25I-Tf. TRV cells ar...

Lycopene ε-cyclase (ε-LCY) is a key enzyme that catalyzes the synthesis of α-branch carotenoids through the cyclization of lycopene. Two cDNA molecules encoding ε-LCY (designated Ntε-LCY1 and Ntε-LCY2) were cloned from Nicotiana tabacum. Ntε-LCY1 and Ntε-LCY2 are encoded by two distinct genes with different evolutionary origins, one originating from the tobacco progenitor, Nicotiana sylvestris,...

PURPOSE To investigate the intersession test-retest variability (TRV) of topography- and threshold-based parameters derived from the Nidek MP-1. DESIGN Prospective observational study. METHODS Sixteen participants with and without central scotoma underwent microperimetry in one eye over three sessions at 1-month intervals in a single institution. We calculated 95% coefficient of repeatabili...

BACKGROUND Frequent painful vaso-occlusive crises (VOCs) were associated with mortality in the Cooperative Study of Sickle Cell Disease (CSSCD) over twenty years ago. Modern therapies for sickle cell anemia (SCA) like hydroxyurea are believed to have improved overall patient survival. The current study sought to determine the relevance of the association between more frequent VOCs and death and...