Abstract Plant growth regulators are chemical substances which govern all the factors of development and within plants. The application plant to crops modifies hormonal balance leading increased yield, enhanced crop tolerance against abiotic stress improved physiological trait crops. Paclobutrazol (PBZ) [(2RS, 3RS)-1-(4-chlorophenyl)- 4, 4-dimethyl-2-(1H-1, 2, 4-trizol-1-yl)-pentan-3-ol], is on...

RNA isolation from ripe fruit can be complicated by high concentrations of sugar and water. These sugars interfere with RNA extraction often resulting in low RNA quality and quantities, and high water concentrations dilute the RNA, making isolation difficult. We report a simple but novel method by which the majority of the excess sugar and water in mature fruit of tomato (Lycopersicon esculentu...

the study was conducted to demonstrate expression profile of cold shock protein genes; cold inducible rna binding protein (cirp) and rna binding motif protein 3 (rbm3) in goats (capra hircus) during different seasons to check the gene expression in different thermal conditions. blood samples were collected from healthy goats of tropical and temperate region during peak winter, moderate season a...

the objective of this study was to determine the effect of estradiol on mrna levels encoding fsh receptor (fshr), lh receptor (lhr), estrogenic and progestagenic enzymes. briefly, follicles between 2 and 5 mm diameter were dissected from the ovaries of adult cows collected from a local abattoir, cells were collected by rinsing the follicle walls with dulbecco modified eagle medium/f12 (dmem/f12...

bladder cancer (bc) ranks the second most common genitourinary tract malignant tumor with high mortality and 70% recurrence rate worldwide. mirnas expression has noticeable role in bladder tumorigenesis. the purpose of this study was to assess mir-200c, mir-30b and mir-141 in tissue samples of patients with bc and healthy adjacent tissue samples and their association with muscle invasion, grade...

The study was conducted to demonstrate expression profile of cold shock protein genes; cold inducible RNA binding protein (CIRP) and RNA binding motif protein 3 (RBM3) in goats (Capra hircus) during different seasons to check the gene expression in different thermal conditions. Blood samples were collected from healthy goats of tropical and temperate region during peak winter, moderate season a...

Background: The present study aimed to investigate the presence of leptin mRNA transcript in epididymal spermatozoa of Holstein cattle by RT-PCR analysis. Materials and Methods: To collect sperm from epididymis, the whole testes of three adult Holstein bulls were quickly removed after slaughtering. Samples were transferred to the laboratory on ice and subsequently the epididymal contents were c...

To the Editor: We compared 2 column-based and an automated magnetic bead separation protocol for extracting circulating DNA and RNA from maternal plasma. We obtained peripheral blood from pregnant women at Prince of Wales Hospital, Hong Kong, and King’s College Hospital, London, with informed consent and institutional ethics approval. Thirty second-trimester samples (median gestational age, 17....