The rapidly hybridizing RNA sequences transcribed in primary hamster embryo cells and in lines of serially propagated hamster cells were compared by the technique of RNA-DNA hybridization-competition. Such sequences are known to be transcribed from reiterated DNA sites. There were no rapidly hybridizing RNA sequences in primary hamster embryo cells that were not also present in transformed cell...

BACKGROUND Increased uptake and metabolism of glucose is a characteristic of malignant transformation. Overexpression of glucose transporters, especially Glut-1, is a common event in human malignancies. To date, little is known about the role of Glut-1 in human prostate cancer (PC). The aim of this study was to investigate the expression of Glut-1 both in PC cell lines and clinical specimens of...

Objective. The high temperature requirement factor A3 (HtrA3) is a serine protease homologous to bacterial HtrA. Four human HtrAs have been identified. HtrA1 and HtrA3 share a high degree of domain organization and are downregulated in a number of cancers, suggesting a widespread loss of these proteases in cancer. This study examined how extensively the HtrA (HtrA1-3) proteins are downregulated...

Primary mammary epithelial cell cultures were established from mammary tissue of lactating and non-lactating goats to assess the expression of beta-casein (CSN2) in vitro. Primary cell cultures were established by enzymatic digestion of mammary tissue and characterized using antibodies against cytokeratin 14, cytokeratin 18, and vimentin. The established primary cell lines in the second passage...

A combined transcriptome and proteome analysis of mouse radiation-induced AMLs using two primary AMLs, cell lines from these primaries, another cell line and its in vivo passage is reported. Compared to haematopoietic progenitor and stem cells (HPSC), over 5000 transcriptome alterations were identified, 2600 present in all materials. 55 and 3 alterations were detected in the proteomes of the ce...

To better understand the progression of epithelial cells from a normal to a malignant phenotype, we have compared various responses of normal, preneoplastic, and neoplastia hamster epidermal cells to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA, but not fresh serum-containing me dium, induced omithine deca rboxy lase (ODC) activity in primary hamster epidermal cells; the c...

The fatal neurodegenerative disorders amyotrophic lateral sclerosis and spinal muscular atrophy are, respectively, the most common motoneuron disease and genetic cause of infant death. Various in vitro model systems have been established to investigate motoneuron disease mechanisms, in particular immortalized cell lines and primary neurons. Using quantitative mass-spectrometry-based proteomics,...

To better understand the progression of epithelial cells from a normal to a malignant phenotype, we have compared various responses of normal, preneoplastic, and neoplastic hamster epidermal cells to the tumor promoter 12-O-tetradecanoylphorbol-13-acetate (TPA). TPA, but not fresh serum-containing medium, induced ornithine decarboxylase (ODC) activity in primary hamster epidermal cells; the com...

Numerous lung cell lines are currently used as in vitro models for pharmacological and toxicological studies. However, no exhaustive report about the metabolic capacities of these models in comparison with those of lung tissues is available. In the present study, we used a high-throughput quantitative real-time reverse transcription-polymerase chain reaction strategy to characterize the express...