AIM To develop a decisional tool to identify the most cost effective process flowsheets for allogeneic cell therapies across a range of production scales. MATERIALS & METHODS A bioprocess economics and optimization tool was built to assess competing cell expansion and downstream processing (DSP) technologies. RESULTS Tangential flow filtration was generally more cost-effective for the lower...

Pseudomonas putida for H2S and Arthrobacter oxydans for NH3 were immobilized with Ca-alginate and packed inside glass columns to form fluidized-bed bioreactors. The feasibility of the lab-scale bioreactor for the treatment of H2S or NH3 was examined. Phosphate salt, being added to the nutrient solution as buffer solution, may chelate with Ca2+ in the Ca-alginate beads, resulting in the disinteg...

The enzymatic synthesis of organic compounds in continuous bioreactors is an efficient way to obtain industrially important chemicals. However, few works have focused on the study of the operational conditions and the bioprocess performance. In this work, the aliphatic ester pentyl octanoate was obtained by direct esterification using a continuous packed bed bioreactor containing the immobilize...

alpha-Pinene biodegradation was evaluated in mesophilic and thermophilic biotrickling filters. The potential of silicone oil for enhancing the removal was evaluated too, at both temperatures. Performance was studied at empty bed residence times between 60 and 14s, and concentrations of 0.06-38.84 gm(-3), with or without silicone oil. Efficiency decreased as the pollutant concentration was incre...

In this study, a pellet scale model was developed for trickle bed reactor utilizing CFD techniques. Drag coefficients were calculated numerically at different velocities and bulk porosities in the case of single phase flow through the dry bed. The simulation results were then compared with the prediction of Kozeny-Carman (K-C) equation. The results indicated that drag coefficients calculated fr...

Despite extensive research on droplet impact behavior superhydrophobic surfaces at high Weber numbers, there remains limited understanding of their dynamics low particularly during the retraction stage. In this study, we investigate dynamic characteristics and reveal a unique re-spreading stage smaller numbers. A critical number to trigger is identified as 4.9. dimensionless analysis force, cha...

One of the most critical barriers to widespread adoption of electric cars is the lack of charging station infrastructure. Although it is expected that a sufficient number of charging stations will be constructed eventually, due to various practical reasons they may have to be introduced gradually over time. In this paper, we formulate a multi-period optimization model based on a flow-refueling ...

We consider a model of occupational choice in large economies where individuals differ in their wealth endowment. Individuals can remain self-employed or engage in productive matches with another individual, i.e., form firms. Matches are subject to a moral hazard problem with limited liability. The division of the gains from such matches is determined by competitive forces. When the incentive p...