We developed a multiplex enzyme-based electrochemical genosensor for sequence-specific detection of multiplex linear-after-the-exponential-PCR amplicons that targeted toxigenic Vibrio cholerae O1 and O139 using novel screen-printed gold electrode bisensors.

Relevance. It is known that antitoxic immunity saves from diphtheria, but does not prevent the carriage of Cd (tox+). Asymptomatic carriers (tox+) play a major role in maintaining epidemiological process diphtheria infection. Aims. Characteristics population composition and specifics kinetic reactions (tox-) simultaneously functioning among people. Results. The functional features specific indi...

The relationship between heat-stable deoxyribonuclease and coagulase production was investigated in the interest of developing more rapid diagnostic and quantitative procedures for distinguishing toxigenic and pathogenic staphylococci from closely related saprophytic organisms.

objective: citrinin is a mycotoxin produced by several species of the genera aspergillus and penicillium and occurs mainly in food products and animal feed. some scientific reports show a link between citrinin and nephrotoxic and possibly a carcinogenic effect for humans. methods: samples collected by settling plates,in northern iran and pure culture isolation performed till the toxin measureme...

introduction: toxigenic clostridium difficile is the major cause of antibiotic-associated diarrhea, colitis, and pseudomembranous colitis. the pathogenicity of c. difficile is related to toxins a&b.; children with cancer are at risk of developing c. difficile infection (cdi) due to increased exposure to antibiotics, immunosuppression, and longer hospital stays. recently, due to higher sensitivit...

risk of Rift Valley fever outbreaks in African savannas using vegetation index time series data. To the Editor: Diphtheria is rare in countries with high vaccination coverage, but as seen in Europe in recent decades, control can disintegrate rapidly. When diphtheria is rare, surveillance is challenging because clinicians have no experience with the infection, and disease may be mild or atypical...

Clostridium difficile is a Gram-positive bacterium commonly found in health care and long-term-care facilities and is the most common cause of antibiotic-associated diarrhea. Rapid detection of this bacterium can assist physicians in implementing contact precautions and appropriate antibiotic therapy in a timely manner. The purpose of this study was to compare the clinical performance of the Qu...

A triplex PCR method was developed to simultaneously amplify a heat-labile toxin sequence (LT) of 258 bp, a shiga-like toxin I sequence (SLT I) of 130 bp, and a shiga-like toxin II sequence (SLT II) of 346 bp from toxigenic strains of Escherichia coli. This method was used to screen 377 environmental E. coli isolates from marine waters or estuaries located in Southern California and North Carol...