Advances in miRNA research have led to the increased demand for techniques appropriate for the analysis of this small RNA subclass. This Application Note describes the use of the Small RNA assay with the Agilent 2100 bioanalyzer to quickly and efficiently analyze and monitor miRNA content in total RNA samples. Catherine Tissot Application Note Analysis of miRNA content in total RNA preparations...

Total RNA and DNA of mimosa epicotyl tissues were extracted and the RNA fractionated into specific soluble RNAs (sRNAs) at different times during late germination. Epicotyls collected at each time contained qualitatively comparable meristematic and developing tissues, while mature tissues increased. Quantitative ratios of total RNA to DNA and total sRNAs to approximated ribosomal RNA (rRNA) var...

Although never demonstrated unequivocally, it has been generally accepted that the largest fraction of the ribonucleic acid of mammalian cells is ribosomal. However, it has been reported recently that about 65% of rat liver RNA has a nonribosomal sedimentation coefficient in the range of 4 S. To resolve the question of the ribosomal RNA content of rat liver and to examine the validity of the as...

The main objective of this study was to isolate high-quality total ribonucleic acid (RNA) from raw fresh semen and frozen-thawed boar semen, using a protocol comprising the conventional TRIzol assay and a membrane-based technique, the PureLink RNA mini kit. Bioanalyzer profile revealed that the sperm RNA size distributions comprised mainly intact RNA ranging from 1500 to 1800 bp, without any de...