The analysis of historical materials is an essential component cultural heritage conservation. Protein was one the most important and pervasive organic substances in ancient human societies. Through qualitative quantitative examination protein-based materials, it possible to clarify their source functions heritages, investigate manufacturing technology heritage, identify deterioration mechanism...

The aim of the present study was to use an enzyme-linked immunosorbent assay (ELISA) to determine the concentrations of Lifeguard (LFG) protein in the serum of 36 patients diagnosed with breast cancer and to compare these values with the concentrations of LFG protein in the serum of 7 healthy volunteers in order to detect a possible association between the expression of LFG in the serum and the...

BACKGROUND Vitamin D deficiency is common in cystic fibrosis (CF), but there is no previous data on free 25-hydroxyvitamin D (25[OH]D) in CF or in relation to healthy individuals. METHODS We assessed total serum 25(OH)D concentration by chemiluminescence and serum free 25(OH)D concentration by both direct measurement (ELISA) and calculation, using serum albumin and vitamin D binding protein (...

PURPOSE Buckwheat is a major cause of anaphylaxis, and Fag e 3 is the key major allergen in buckwheat. However, an immunoassay system for the quantification of Fag e 3 has yet to be developed. METHODS We developed a 2-site enzyme-linked immunosorbent assay (ELISA) using monoclonal antibodies (mAbs) produced against recombinant Fag e 3. We applied this ELISA to quantify native Fag e 3 in total...

one of the inflammatory mediators which is secreted by inflammatory cells is high-mobility group protein b1 (hmgb1). interaction of hmgb1 and toll-like receptors (tlrs) leads to increased production of inflammatory cytokines. on the other hand, it was shown that triggering receptor expressed on myeloid cells (trem-1) also can be activated by tlrs, and its soluble form (strem-1) can be formed by...

Background: The mechanism of para-nonylphenol (PNP) reducing the proliferation and differentiation of bone marrow mesenchymal stem cells (MSCs) is not known. The present study was designed to investigate the mechanism. Methods: MSCs were extracted under sterile condition from Wistar rat and cultured in DMEM, containing 15 % FBS and penicillin/streptomycin until the 3rd passage, then cells we...

INTRODUCTION: Leptin is a hormone resulting from the obesity gene which could be important in the pathogenesis of the insulin. Only limited data concerning the interaction of insulin, glucose tolerance and free leptin are available and no data exist on the potential influence of free vs. bound circulating leptin. We, therefore, studied percentage of free to total leptin in diabetic patients. ...

Purpose: To evaluate colostrum total protein differences between left and right breasts and between mothers of boy and girl infants. Materials and Methods: A cross sectional study for delivery was carried out on 30 boy-delivered and 30 girl-delivered mothers aged 16 to 36 years, conducted at the Izadi and Al-Zahra Hospitals in Qom city, Iran. Colostrum was collected from both breasts by man...