Protoplasts, RNA, Plant Viruses, Tobacco Mosaic Virus, Polyethylene Glycol Infection of tobacco protoplasts with R N A of tobacco mosaic virus and the bromoviruses has been studied. The quality of the R N A was very important. Ribonuclease activity was controlled by incorporating 5 mM ZnCl2 in the inoculum. Up to 60% infection has been observed with bromovirus R N A but infection with TM V R N ...

Two morphactins and three aminofluorenes initiated the formation of compact tissue nodules in hormone-dependent tobacco callus cultures. These nodules, upon subculture, behaved like partially transformed plant tumors. They grew on cytokinin-free media, while control callus and nonnodule tissue still required an exogenous cytokinin source. The data indicate that substituted fluorenes, including ...

We report the production of hGM-CSF cytokine in leaves of industrial tobacco cultivars DH-17 and DH-27 by using Agrobacterium-mediated transient expression. We prove the concept that very high biomass industrial tobacco plants are suitable platforms for rapid, low cost production of foreign proteins. Successful transient expression of the GM-CSF was achieved in less than three months, opening t...

Cultured tobacco plant cells activated 2-aminofluorene to an agent mutagenic to Salmonella typhimurium strain TA98. The plant activation of 2-aminofluorene is heat-inactivated and may not involve solely cytochrome P-450. The kinetics of activation demonstrated both time- and concentration-dependent responses.

The great potential of pharmacologically active secondary plant metabolites is often limited by low yield and availability of the producing plant. Chemical synthesis of these complex compounds is often too expensive. Plant cell fermentation offers an alternative strategy to overcome these limitations. However, production in batch cell cultures remains often inefficient. One reason might be the ...

Tobacco mosaic virus (TMV) has had an illustrious history for more than 100 years, dating to Beijerinck's description of the mosaic disease of tobacco as a contagium vivum fluidum and the modern usage of the word "virus." Since then, TMV has been acknowledged as a preferred didactic model and a symbolic model to illuminate the essential features that define a virus. TMV additionally emerged as ...

The transcription factor DREB1A/CBF3 specifically interacts with the dehydration responsive element (DRE/CRT) and induces expression of genes involved in environmental stress tolerance in Arabidopsis: Overexpression of DREB1A improved drought- and low-temperature stress tolerance in tobacco. The stress-inducible rd29A promoter minimized the negative effects on the plant growth in tobacco. Furth...

In an effort to combat the risks associated with traditional tobacco products, product innovation has been redirected towards reducing consumer’s potential exposure harmful or potentially constituents (HPHCs). Among these innovations are modern oral nicotine products (MONPs). This class aims deliver while limiting toxicant exposure. body of work sought investigate for select HPHC (tobacco-speci...

medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. P lan ta rum 15:473-497. 17. Sawhney, R., and J. M. Webster. 1975. T h e role of plant growth hormones in determining the resistance of tomato plants to the root-knot nematode, Meloidogyne incognita. Nematologica 21:95103. 18. Vasil, I. K., M. R. Ahuja, and V. Vasil. 1979. Plant tissue cultures in genetics and plant br...

BACKGROUND Hepatitis C virus (HCV) is major cause of liver cirrhosis in humans. HCV capsid (core) protein (HCVcp) is a highly demanded antigen for various diagnostic, immunization and pathogenesis studies. Plants are considered as an expression system for producing safe and inexpensive biopharmaceutical proteins. Although invention of transgenic (stable) tobacco plants expressing HCVcp with pro...