The content of ribo- and desoxyribonucleic acids in chick heart cultures at regular intervals after implanting has been determined both with standard culture medium and with Tyrode's solution alone. With an inadequate medium, both nucleic acids dropped in a consistent manner but ribonucleic acid was affected to a much greater extent. When the medium was adequate for growth, both fractions rose ...

tissue culture came after 19 years in a job I loved at a small pharmaceutical company (although in this first case no one actually offered me any tissues). Having been at the company for such a long time, I felt I had some job security. But I found out how wrong I was when the company was sold. Part of me understood that losing my job this way was “just business,” but I still felt a deep profes...

Observations on mouse monocytes, maintained in cell culture and inoculated with Histoplasma capsulatum, have been previously reported (Howard, 1959). H. capsulatum differs from other fungi pathogenic for man in that it is primarily a parasite of cells of the reticulo-endothelial system. Thus the fungus was ideally suited to preliminary studies on the usefulness of tissue culture techniques for ...

The propagation of mammalian cells in stainless steel fermentors of a type used in antibiotic fermentations represents a marked departure from the techniques employed in classical tissue culture studies. Since the pioneer work of Harrison (1907), in which he demonstrated the outgrowth of cells from nerve tissue maintained in vitro, many techniques for the in vitro culture of cells have been dev...

The propagation of mammalian cells in stainless steel fermentors of a type used in antibiotic fermentations represents a marked departure from the techniques employed in classical tissue culture studies. Since the pioneer work of Harrison (1907), in which he demonstrated the outgrowth of cells from nerve tissue maintained in vitro, many techniques for the in vitro culture of cells have been dev...

plates 16. 45s.). Edinburgh and London: E. & S. Livingstone, 1970. IT is now more than ten years since Dr. Paul introduced from the Tissue Culture Association's Summer School the idea, which is now well realised, that temporary or continuous multiplication of mammalian cells is practical laboratory politics. Since then his book has come to the fourth edition, and the unaltered arrangement of th...

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